Sequences from confirmation amplicon


Confirmation sequences for line 285E04 and hit At4g27960 are shown.
Line ID 285E04
Locus At4g27960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13916181
Primers used for PCR 09wz - 8474, show primer details
   
Sequence Name 91-K118926-0022-285-E04C-09wz
GenBank Accession LN515147 [GenBank]
Sequencing primer gene specific primer, 09wz: 5'-CTGGTTTGGTATTGAAGTTTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K118926-0022-285-E04C-09wz
CAGGCCACTATAATGGGTCCATCGGATAGCCCTTATTCTGGAGGAGTTTTTCTTGTAACC
ATCCATTTCCCTCCAGATTACCCATTTAAGCCTCCTAAGGTATATTCAAATCTCTACAGC
TGCTTCTTCTTCTTTGAATTTTTTTTCCAACTTTACTTCGGCCATCATTTTTCTGTTGCT
AATATCTTCCATCGTTTATCTATTGTTCTTTTCTCTGTGTTCTTGATTTCATTGCTCTCT
ATGTATGTTGTTACAAAAAATGTAGGTGGCTTTTAGGACGAAGGTGTTCCATCCAAACAT
TAACAGCAATGGAAGCATCTGCCTCGACATCTTGAAGGAGCAGTGGAGTCCTGCTCTCAC
AATTTCCAAGGTTTTCTTTTGTTCTTCCTCATCTTTATCATATTAGAACCATAATTTTGC
AGCTTTGCTCTCCAGGTGACATCAATTTTGTGTGGTGCAACAGGTGCTGCTATCGATCTG
TTCTTTGTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGGTTATT
   
Sequence Name 67-K118925-0022-285-E04C-8409
GenBank Accession LN511915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118925-0022-285-E04C-8409
TTCATTAACAAAGAACAGATCGATAGCAGCACCTGTTGCACCACACAAAATTGATGTCAC
CTGGAGAGCAAAGCTGCAAAATTATGGTTCTAATATGATAAAGATGAGGAAGAACAAAAG
AAAACCTTGGAAATTGTGAGAGCAGGACTCCACTGCTCCTTCAAGATGTCGAGGCAGATG
CTTCCATTGCTGTTAATGTTTGGATGGAACACCTTCGTCCTAAAAGCCACCTACATTTTT
TGTAACAACATACATAGAGAGCAATGAAATCAAGAACACAGAGAAAAGAACAATAGATAA
ACGATGGAAGATATTAGCAACAGAAAAATGATGGCCGAAGTAAAGTTGGAAAAAAAATTC
AAAGAAGAAGAAGCAGCTGTAGAGATTTGAATATACCTTAGGAGGCTTAAATGGGTAATC
TGGAGGGAAATGGATGGTTACAAGAAAAACTCCTCCAGAATAAGGGCTATCCGATGGACC
CATTATAGTGGCCTGCCAATGAAACATGTCTTCCGCAACGGGTCCTGTCAACATTTAACC
AAAACTTCAATACCAAACCAG

Last Updated on 06.12.2018 16:11