Sequences from confirmation amplicon


Confirmation sequences for line 285E05 and hit At3g59100 are shown.
Line ID 285E05
Locus At3g59100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21854071
Primers used for PCR VL26 - 8474, show primer details
   
Sequence Name 35-K020889-022-285-E05C-8409
GenBank Accession LN501653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K020889-022-285-E05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTTGAATAATAATA
TTATTAAAGACCATATGTCAAAATCATTGTGATAACTACAACACATGAGTGTGATGAGAG
TGTGAGGTGTCCAAGTCCAACCAAACTAAAATATCCCTATAACCTTATAGACATCTATTG
AACTACGCTAAAACGACACCGTTCTAAAGAAGCAGGTTTGTTGCTTAAGACAGAAGAAAC
CCGGAAACAACACTGAGTATTTATGGTTTTACTTGTGCGAAGAAGTTGCTTTGTCTTTCC
GACCAGCGAGAATCATAGAGATTTGAAGGCCTCTGCTAAAAGCTTGATTGAAAAGCAGAC
GAGCTTGAAACTCGGAGACAATGGGAAACCAAGACAGAACAGCGATTGGTGCAAAGATTA
CAAGTCCCATGATATTCTCATACGCTCTTCCTAGCTC
   
Sequence Name 36-K020889-022-285-E05C-VL26
GenBank Accession LN502051 [GenBank]
Sequencing primer gene specific primer, VL26: 5'-AGAGCTAGGAAGAGCGTATGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K020889-022-285-E05C-VL26
TATCATGGGACTTGTAATCTTTGCACCAATCGCTGTTCTGTCTTGGTTTCCCATTGTCTC
CGAGTTTCAAGCTCGTCTGCTTTTCAATCAAGCTTTTAGCAGAGGCCTTCAAATCTCTAT
GATTCTCGCTGGTCGGAAAGACAAAGCAACTTCTTCGCACAAGTAAAACCATAAATACTC
AGTGTTGTTTCCGGGTTTCTTCTGTCTTAAGCAACAAACCTGCTTCTTTAGAACGGTGTC
GTTTTAGCGTAGTTCAATAGATGTCTATAAGGTTATAGGGATATTTTAGTTTGGTTGGAC
TTGGACACCTCACACTCTCATCACACTCATGTGTTGTAGTTATCACAATGATTTTGACAT
ATGGTCTTTAATAATATTATTATTCAAAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11