Sequences from confirmation amplicon


Confirmation sequences for line 285E06 and hit At3g57880 are shown.
Line ID 285E06
Locus At3g57880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21432162
Primers used for PCR KN31 - 8474, show primer details
   
Sequence Name 38-K103252-0022-285-E06C-8409
GenBank Accession LN502942 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103252-0022-285-E06C-8409
ATTTCCCGATTCTTGAATCTTTTGCTCCTCCAATCTTCTCACCTCCATGGAGATGGCAGT
TATCAAAAACTCCAACAGTAACAACGGTGCACGGATCAAAAACCTCCCAAGTATATTGCT
CATTCCATCTTGGTGTAAAGCTGTCAATGATTGTCCGAGTTCGAATCCATTTCTGTCCGT
ACTTCGCCACACAATATGCATCTGTGGTTCCCCGGCCGTCTTTGGTTTTCATAGGCATCA
GACCTGTAGCATTCAGTATCCCCAACTCCAGTACACCGATNTTAGGCTTCCATAGTTGCT
TCGCTGTTGGTCTAAGATCACTGCTGTAATGCGTAGACTCATCGAGAACGTGATACCCTC
CTTCCAAACATATTCTCATGTGAATCCTGCTAGCGAATTTGGTCTCCTTCTTCTCTCCAT
CGACCATAATATGCTTCTCGAGATTGTACCACCTGCTGTTCACCGGTTTATGGTCAAATC
TTCTGTCCAAATACAGC
   
Sequence Name 86-K103253-0022-285-E06C-KN31
GenBank Accession LN514647 [GenBank]
Sequencing primer gene specific primer, KN31: 5'-TGCAGTATTTGGACAGAAGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K103253-0022-285-E06C-KN31
TCTCGAGAAGCTATTATGGTCGATGGAGAGAAGAAGGAGACCAAATTCGCTAGCAGGATT
CACATGAGAATATGTTTGGAAGGAGGGTATCACGTTCTCGATGAGTCTACGCATTACAGC
AGTGATCTTAGACCAACAGCGAAGCAACTATGGAAGCCTAATATCGGTGTACTGGAGTTG
GGGATACTGAATGCTACAGGTCTGATGCCTATGAAAACCAAAGACGGCCGGGGAACCACA
GATGCATATTGTGTGGCGAAGTACGGACAGAAATGGATTCGAACTCGGACAATCATTGAC
AGCTTTACACCAAGATGGAATGAGCAATATACTTGGGAGGTTTTTGATCCGTGCACCGTT
GTTACTGTTGGAGTTTTTGATAACTGCCATCTCCNTGGAGGTGAGAAGATTGGAGGAGCA
AAAGATTCAAGAATCGGGAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAAT

Last Updated on 06.12.2018 16:11