Genotyping and primer details

Genotyping and primer details for 285E06 - At3g57880
Line ID 285E06
Locus At3g57880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21432162 of line 285E06 Chr3:21432162
Insertion allele amplicon 8474 - KN31
Locus specific primer KN31: 5'-TGCAGTATTTGGACAGAAGATTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21432657
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11