Sequences from confirmation amplicon


Confirmation sequences for line 285F11 and hit At2g46380 are shown.
Line ID 285F11
Locus At2g46380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19039437
Primers used for PCR 03ih - 8474, show primer details
   
Sequence Name 31-K112776-0022-285-F11C-03ih
GenBank Accession LN500234 [GenBank]
Sequencing primer gene specific primer, 03ih: 5'-TTCTTTTATTGTTGTCCCAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112776-0022-285-F11C-03ih
TTTCTCCTCTTTGATGATGTTTTTTTTCGCGTTAATTTATTCCAGTAATTTCCCTTAGTT
TAAACATTACAACACTAACTACTAAATGTAAGTAAAAAAGTAAAGGTAAATCTTTAAATT
TTATTCCTTGGCTGAAAAAGAAAGAAAGTTACGAGTTACAAAAATGGGCCCACTTTGTAG
ATTGGCCCGCCCAAAATAAATCGATTTGAAACTCAATTTTGTATAGTCAGTCTCTCTCTT
CTTCATGTTTGGTTTCAGAGAATGAAAGGCTTAACAAGAGCTTCTTCATTGACTCCATCT
TCTGCATCTCTTTAGAGTTTATAGATCTAATAACAGCAATAAATCTAGCTTCTTAAGTCT
TCTTGGTTTTTCTTCTTTAATTGAATGCTGAGTATCTTTCTTGTCAGCTCAGTGACTATT
TTCGTTTGTTTGTAATGGCTGAGTCGTCAACGATCGAAGCAAGGCTGGTTCGATGTCCCA
AGTGCAAGAATCTCTTAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 08-K112774-0022-285-F11C-8409
GenBank Accession LN489803 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112774-0022-285-F11C-8409
GCATTTACATTTAGAGATTCTTGCACTTGGGACATCGAACCAGCCTTGCTTCGATCGTTG
ACGACTCAGCCATTACAAACAAACGAAAATAGTCACTGAGCTGACAAGAAAGATACTCAG
CATTCAATTAAAGAAGAAAAACCAAGAAGACTTAAGAAGCTAGATTTATTGCTGTTATTA
GATCTATAAACTCTAAAGAGATGCAGAAGATGGAGTCAATGAAGAAGCTCTTGTTAAGCC
TTTCATTCTCTGAAACCAAACATGAAGAAGAGAGAGACTGACTATACAAAATTGAGTTTC
AAATCGATTTATTTTGGGCGGGCCAATCTACAAAGTGGGCCCATTTTTGTAACTCGTAAC
TTTCTTTCTTTTTCAGCCAAGGAATAAAATTTAAAGATTTACCTTTACTTTTTTACTTAC
ATTTAGTAGTTAGTGTTGTAATGTTTAAAACTAAGGGAAATTACTGGAATAAATTAACGC
TGAAAAAAAACATCAATCAAAGAGGGAGAAAAGTCAAGGTTAAGGTCAAAAGCCAAACTG
GTTTTGGGACAACATTTAAAAGA

Last Updated on 06.12.2018 16:11