Sequences from confirmation amplicon


Confirmation sequences for line 286A12 and hit At5g27310 are shown.
Line ID 286A12
Locus At5g27310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9625504
Primers used for PCR FV92 - 8474, show primer details
   
Sequence Name 06-K100894-0022-286-A12C-8409
GenBank Accession LN488729 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100894-0022-286-A12C-8409
ATTTACATGTATTCCGGACATAGCATGGGTTCCGTGTATTGGTAATTTCAGTATCTCTCC
TTGGCAATTCTCACTCTCATCATCTTTCCGTCGTTCTTGATCATCAGGTGGCTAGGGTTT
TCAGTTTATGTTGAAATCGAAATATGAAAGAGATAATGAACAAGAGTAAATATATAGGAG
GACTTGTGAGCGGTGCCTTACAAAAAGGAAACTTGAGTAACGTGAGATTCAAGGATTAGA
TTTGGATTTGAATTTTCTTATGAGATATAAAGATAGAAGATATACCGAGAGTTGTAACAA
ATCCACATGTAAACAAATCAAAATCATTACAATTTGTCTAGTACAATTTTGGTTTGGATG
GGTTGATTTGCTTCTGAAGCAAGAAATAGAGATTGGACCAATAAAGACCAGTTACTGATT
TCAGGACTGGAACAAGTTTTCAAGATTGGCACCAATTACCATATTCACAATCTTGCAACT
TGCAAGAACACAAATTATATGATCAAGTAGTAAACAAGGTTTTCCATTAGCTG
   
Sequence Name 06-K100902-0022-286-A12C-FV92
GenBank Accession LN488730 [GenBank]
Sequencing primer gene specific primer, FV92: 5'-TGTTTGGTATTGCTCAGCTAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K100902-0022-286-A12C-FV92
ATATAATTTGTGCTCTTGCAGTTGCAAGATTGTGAATATTGTAATTTGTGCCTATCTTGA
AAACTTGTTACAGTCCTGAAATCAGTAACTTGTCTTTATTTGTCCAATCTCTATTTCTTG
CTTCAGAAGCAAATCAAACCATCCAAACCAAAATTGTACTAGACAAATTGTAATGATTTT
GATTTGTTTACATGTGGATTTGTTACAACTCTCGGTATATCTTCTATCTNTATATCTCAT
AAGAAAATTCAAATCCAAATCTAATCCTTGAATCTCACGTTACTCAAGTTTCCTTTTTGT
AAGGCACCGCTCACAAGTCCTCCCATATATTTACTCTTGTTCATTATCTCTTTCATATTT
CGATTTCAACATAAACCGAAAACCCTAGCCACCTTATGATTAAGAACGACCGAAAGATTA
TTAAAGTGAGAATTGCCCAGGAAAAATACTGAAATTACCAATACACGGAACCCATGCTAT
GTCCCGAATACATGTAAATGGCTTCATGTCCGGGAAATCTACCTGGATTAACAATGAGTA
TGAAGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11