Sequences from confirmation amplicon


Confirmation sequences for line 286D03 and hit At5g54920 are shown.
Line ID 286D03
Locus At5g54920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22305549
Primers used for PCR 11sf - 8474, show primer details
   
Sequence Name 37-K116569-0022-286-D03C-11sf
GenBank Accession LN502750 [GenBank]
Sequencing primer gene specific primer, 11sf: 5'-TCATGTTTGATTTGTCTTAGGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K116569-0022-286-D03C-11sf
ATTTTTCTCAGGCCATTGATCTTCTGAAACTATTATTTCTTCTTGCAAACCCTTAAGAGC
TTAAAGTCTGATTCAATCTTATTGTAATCAGAAATAGGAAAACAGAATCTAACTATTGTC
TAGCTTAAAGATGTCAGCAAAATTATCGTGATTCATTATATAAAATGCTCATAACTTCCT
CTTTCTGTTTTCTTTAGCTAGTAGTTGTATATCATTGAGAAAGAAAAAAAATCCAAGTCA
TCAGACGGATCTATAATTTTTGGTTTTTAACTATGTATCAAAAACTTATTAAGCTGGGAG
GCAAATAATAAGGTAATGAAGATGAAAAACAAGACTTTCTATAGAAACTTTGTGCCATAG
AAACCAGAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCA
   
Sequence Name 08-K116568-0022-286-D03C-8409
GenBank Accession LN489894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116568-0022-286-D03C-8409
CATTTACATTGGTAATTCTGGTTTCTATGGCACAAAGTTTCTATAGAAAGTCTTGTTTTT
CATCTTCATTACCTTATTATTTGCCTCCCAGCTTAATAAGTTTTTGATACATAGTTAAAA
ACCAAAAATTATAGATCCGTCTGATGACTTGGATTTTTTTTCTTTCTCAATGATATACAA
CTACTAGCTAAAGAAAACAGAAAGAGGAAGTTATGAGCATTTTATATAATGAATCACGAT
AATTTTGCTGACATCTTTAAGCTAGACAATAGTTAGATTCTGTTTTCCTATTTCTGATTA
CAATAAGATTGAATCAGACTTTAAGCTCTTAAGGGTTTGCAAGAAGAAATAATAGTCTTC
AGAAGATCAATGGCCTTGAGAAAAATGGGTCTCTCTCACATTCTCCTGCACAGCCTAAGA
CAAACAAAACATG

Last Updated on 06.12.2018 16:11