Sequences from confirmation amplicon


Confirmation sequences for line 286G01 and hit At1g68600 are shown.
Line ID 286G01
Locus At1g68600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25759746
Primers used for PCR 44fm - 8474, show primer details
   
Sequence Name 27-K111117-0022-286-G01C-44fm
GenBank Accession LN498445 [GenBank]
Sequencing primer gene specific primer, 44fm: 5'-GAATTTACTAGCATCTTGAAGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K111117-0022-286-G01C-44fm
AAGCAATTCCCATCTTGATGGAGAAGTAAAGTTTCCTACGATCAGAATGTCCCAGCGCGT
ACAACTTCGCCGGAGCCTCGTAGAGAGCCCTCCACGAGCAACACCGGAACTTGGAGTCAC
GGCTATCAGCGACGTCGCTTGACTGCCAGAGAAGCTTCTCTGTGTTTTGCTCCGGTACTG
ATCCCATTTTACCTCCCATTAGAAATGATTCTGACAATTCTACAGAACCTGAGAGATTCT
TGTCGTCCTTACGAATAGAAAGATGTTTATTTATCTATTTATCAAGATTTGTTTTGTACT
ACGCAATTAATGCATGCCTTCGATTCATCATCTCTAAATAATCAAGTTTTTTTAAAAAAT
TTACAATTTGGTGCATCTACATTACAAATTTTAATAATTGAAATCTAATCATTACTAATC
AATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 11-K111116-0022-286-G01C-8409
GenBank Accession LN491172 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K111116-0022-286-G01C-8409
ATTGATTATAATGATTAGATTTCAATTATTAAAATTTGTAATGTAGATGCACCAAATTGT
AAATTTTTTAAAAAACTTGATTATTTAGAGATGATGAATCGAAGGCATGCATTAATTGCG
TAGTACAAAACAAATCTTGATAAATAGATAAATAAACATCTTTCTATTCGTAAGGACGAC
AAGAATCTCTCAGGTTCTGTAGAATTGTCAGAATCATTTCTAATGGGAGGTAAAATGGGA
TCAGTACCGGAGCAAAACACAGAGAAGCTTCTCTGGCAGTCAAGCGACGTCGCTGATAGC
CGTGACTCCAAGTTCCGGTGTTGCTCGTGGAGGGCTCTCTACGAGGCTCCGGCGAAGTTG
TACGCGCTGGGACATTCTGATCGTAGGAAACTTTACTTCTCCATCAAGATGGGAATTGCT
TTGGCACTCTGTTCTTTCGTCATCTTTCTCAAAGAGCCGCTTCAAGATCTGNNTAAATT

Last Updated on 06.12.2018 16:11