Sequences from confirmation amplicon


Confirmation sequences for line 286G01 and hit At3g24620 are shown.
Line ID 286G01
Locus At3g24620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8982153
Primers used for PCR MJ31 - 8474, show primer details
   
Sequence Name 18-K029910-022-286-G01C-8409
GenBank Accession LN494176 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K029910-022-286-G01C-8409
CTTTCTTTACATTGTCGAGTTTGTTCCTTCTCAACAAACAAGCAAAGATGGAGTTTGCAC
TGAGGTTTGAGTTTCCCAAAGAAAAGAAAATATCATTTTTGCGACCTCTTTAGAATAAGT
TACAATGTTAACCATTTTTTTGTAGATTATGGTGACAAGACAAAGAGGTGATCTTCTTAT
GAACATTCCAGCTCTTCGAAAACTTGACGCCATGCTCATCGTAAGTCTCTCCATTCAATC
TGTATTGAATCCGGCTTATATCTAGCGTTTGGTGGATTAATGGTAATGATAACATGTTCA
GGATACATTGGACAATTTCAGAGGACACAACGAGTTTTGGTATGTCTCAAGAGATTCAGA
AGAAGGGCAGCAAGCAAGGAATGATCGGACAAACGACAAGTGGTGGCTACCACCGGTTAA
AGTTCCTCCTGGAGGTTTATCAGAACCATCTCGGAGAATGCTTTACTTCCAAAAAGATTC
AGTCACACAAGTTCAAAAAGCTGCAATGGCCATCAATGCTCAAGTCCTCT
   
Sequence Name 18-K029910-022-286-G01C-MJ31
GenBank Accession LN494177 [GenBank]
Sequencing primer gene specific primer, MJ31: 5'-ATTTCCATTTCTGAGAGGACTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K029910-022-286-G01C-MJ31
GCTTTTTGACTTGTGTGACTGAATCTTTTTGGAAGTAAAGCATTCTCCGAGATGGTTCTG
ATAAACCTCCAGGAGGAACTTTAACCGGTGGTAGCCACCACTTGTCGTTTGTCCGATCAT
TCCTTGCTTGCTGCCCTTCTTCTGAATCTCTTGAGACATACCAAAACTCGTTGTGTCCTC
TGAAATTGTCCAATGTATCCTGAACATGTTATCATTACCATTAATCCACCAAACGCTAGA
TATAAGCCGGATTCAATACAGATTGAATGGAGAGACTTACGATGAGCATGGCGTCAAGTT
TTCGAAGAGCTGGAATGTTCATAAGAAGATCACCTCTTTGTCTTGTCACCATAATCTACA
AAAAAATGGTTAACATTGTAACTTATTCTAAAGAGGTCGCAAAAATGATATTTTCTTTTC
TTTGGGAAACTCAAACCTCAGTGCAAACTCCATCTTTGCTTGTTTGTTGAGAAGGAACAA
ACTCGACAATGTAAATGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11