Sequences from confirmation amplicon


Confirmation sequences for line 287D02 and hit At3g20010 are shown.
Line ID 287D02
Locus At3g20010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6973544
Primers used for PCR HP19 - 8474, show primer details
   
Sequence Name 17-K018155-022-287-D02C-HP19
GenBank Accession LN493662 [GenBank]
Sequencing primer gene specific primer, HP19: 5'-GTCTGCTGCTTGGATATTTACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K018155-022-287-D02C-HP19
TAACGTCTACTCTGCAGGGACTTGGTAAGACGGTCTCGACGATTGCTCTTATCCTAAAGC
AAAAGATTGTGTCACAACTGAAGTCCGAAAGTTCATGCAAGCAAGAAACTGAGGCATTAG
TCCTGGATGCTGATGATGAGTCAGACAATGCAAAGCATGAAAGTGGTAGTCATGTAAAAC
CAGAGCTCAAGGTTTCAAGCAACAGTGAGACCTCGGTTCTGAGTGCATGTGGTAATGATG
AGAATGACAGTTCAGATATGGAGAAAGCCGAAGATGAAGAAGCTAATTCTTCAACACGAG
CATTTCAGTGGAAGAGACCAGCTGCTGGTACATTAATTGTTTGCCCAGCGAGTGTTGTAA
GGCAGTGGGCAAGAGAGCTTGATGAGAAGGTTTCTGAAGAATCCAAACTTTCTGTGTTAG
TCTACCATGGTAGTAATAGGACCAAAGATCCTAATGAATCAGCAATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACNAATTACGATCCGTCG
   
Sequence Name 18-K018155-022-287-D02C-8409
GenBank Accession LN494106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K018155-022-287-D02C-8409
TAGATTTCCCGGACATTGCTGATTCATTAGGATCTTTGGTCCTATTACTACCATGGTAGA
CTAACACAGAAAGTTTGGATTCTTCAGAAACCTTCTCATCAAGCTCTCTTGCCCACTGCC
TTACAACACTCGCTGGGCAAACAATTAATGTACCAGCAGCTGGTCTCTTCCACTGAAATG
CTCGTGTTGAAGAATTAGCTTCTTCATCTTCGGCTTTCTCCATATCTGAACTGTCATTCT
CATCATTACCACATGCACTCAGAACCGAGGTCTCACTGTTGCTTGAAACCTTGAGCTCTG
GTTTTACATGACTACCACTTTCATGCTTTGCATTGTCTGACTCATCATCAGCATCCAGGA
CTAATGCCTCAGTTTCTTGCTTGCATGAACTTTCGGACTTCAGTTGTGACACAATCTTTT
GCTTTAGGATAAGAGCAATCGTCGAGACCGTCTTACCAAGTCCCTGCAGAGTAGACGTTA
CATAGTTTGAGGTAAATATCC
Primers used for PCR HP20 - 8474, show primer details
   
Sequence Name 13-K018334-022-287-D02C-HP20
GenBank Accession LN491829 [GenBank]
Sequencing primer gene specific primer, HP20: 5'-GCAGCATTATAGCCCTTAGAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K018334-022-287-D02C-HP20
CTTCTTGTAACCTTGACAAGAATTTCTGGAAATTGGAACCTTGATTGTACTGTAAAATGA
CTTGTACACCGCATATGGATCATATCTAAGAAACCTGAAATAGCTATATAAATCATCAAT
CGTATTTTGTATTGGTGTTCCAGACAAGCACCACCTCCTTTTGGCTCGAAGAGTGCAACA
TGATCTTGCCATTTGTGTTCTATAGTTCTTAATTGTCTGAGCTTCATCTAGTACTATTCT
GAACCACCCAACCTTTCCTAGAGGACCACAATCAGGCTCAGAAGAAGTATCGTTAGTGCT
TTTTCTACCTCTTCTTTTACTCTTCTTACTAGCACCCACCACGACTTTCCGTTTCTTATT
GTTGGAGAAGCCAGAGGCAAGACCATATCTGTCAGTATTTTTTTCATCGTTCTCATCCTC
ATCCACCAAGAATTTGTTGGGAGCTTCATTAGTAACAATAGCGTACGTTGTCACAACCAC
ATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCT
   
Sequence Name 14-K018334-022-287-D02C-8409
GenBank Accession LN492295 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K018334-022-287-D02C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACATGTGGTTGTGACAACGTACGCTATT
GTTACTAATGAAGCTCCCAACAAATTCTTGGTGGATGAGGATGAGAACGATGAAAAAAAT
ACTGACAGATATGGTCTTGCCTCTGGCTTCTCCAACAATAAGAAACGGAAAGTCGTGGTG
GGTGCTAGTAAGAAGAGTAAAAGAAGAGGTAGAAAAAGCACTAACGATACTTCTTCTGAG
CCTGATTGTGGTCCTCTAGGAAAGGTTGGGTGGTTCAGAATAGTACTAGATGAAGCTCAG
ACAATTAAGAACTATAGAACACAAATGGCAAGATCATGTTGCACTCTTCGAGCCAAAAGG
AGGTGGTGCTTGTCTGGAACACCAATACAAAATACGATTGATGATTTATATAGCTATTTC
AGGTTTCTTAGATATGATCCATATGCGGTGTACAAGTCATTTTACAGTACAATCAAGGTT
CCAATTTCCAGAAATTCTTGTCAAGGTTACAAGAAGCTTCAAGCTGTTCTAAGGGC

Last Updated on 06.12.2018 16:11