Genotyping and primer details


Genotyping and primer details for 287D02 - At3g20010
Line ID 287D02
Locus At3g20010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6973544 of line 287D02 Chr3:6973544
   
Insertion allele amplicon 8474 - HP19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP19: 5'-GTCTGCTGCTTGGATATTTACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6973041
   
Insertion allele amplicon 8474 - HP20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP20: 5'-GCAGCATTATAGCCCTTAGAACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6974056
   
wt amplicon (over InsPos) HP20 - HP19
wt amplicon start Chr3:6973041
wt amplicon end Chr3:6974056
wt amplicon size 1015 bp
Locus specific primer HP19: 5'-GTCTGCTGCTTGGATATTTACCTC-3'
2nd locus specific primer HP20: 5'-GCAGCATTATAGCCCTTAGAACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11