Sequences from confirmation amplicon


Confirmation sequences for line 287G07 and hit At1g21400 are shown.
Line ID 287G07
Locus At1g21400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7494141
Primers used for PCR 08wu - 8474, show primer details
   
Sequence Name 44-K119432-0022-287-G07C-08wu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 08wu: 5'-CGCACGAGTCTTTTAATTGTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K119432-0022-287-G07C-08wu
AGCAGATGCTTCGGTTTAAGGCGTTTCATCCGGTTAATCATTCCCAATTTTGGATTTCTT
GGTTTATAAGCTAAATTTAAACCAAATTTAGGTGAAACTGGTTGCCTAGGATCAAAAATT
CTTGCAATGAGTCTCAAAAACTCGAACGTAGAAAAGATATTAAAGATTGAAGATTATTCA
GTCTTAGAAAGTTAGAATTATTCAAATTTACTTGGTGTGTTGGTTTTGATCCGGTATGGG
ATGATCGCATAGTACAACAATATGCAAAAGTGCATAGTACTTCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAGG
TTATT
   
Sequence Name 04-K119431-0022-287-G07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119431-0022-287-G07C-8409
ATTTCATTGAATATATCCTGAAGTACTATGCACTTTTGCATATTGTTGTACTATGCGATC
ATCCCATACCGGATCAAAACCAACACACCAAGTAAATTTGAATAATTCTAACTTTCTAAG
ACTGAATAATCTTCAATCTTTAATATCTTTTCTACGTTCGAGTTTTTGAGACTCATTGCA
AGAATTTTTGATCCTAGGCAACCAGTTTCACCTAAATTTGGTTTAAATTTAGCTTATAAA
CCAAGAAATCCAAAATTGGGAATGATTAACCGGATTGAAACGCCTTTAAACCGAAGCATC
TGCTTTCAGAAGAACTAATTATTTCTTTCTTTACACAATTAAAAGACTCGTGCG

Last Updated on 06.12.2018 16:11