Sequences from confirmation amplicon


Confirmation sequences for line 288B07 and hit At3g48710 are shown.
Line ID 288B07
Locus At3g48710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18041274
Primers used for PCR 02fj - 8474, show primer details
   
Sequence Name 54-K111269-0022-288-B07C-02fj
GenBank Accession LN508516 [GenBank]
Sequencing primer gene specific primer, 02fj: 5'-ATCAGCTTTCTCCTTTATTCACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K111269-0022-288-B07C-02fj
CCACGACTAGCAGATACTGTTGCTGGTTACCGAGAAGGACACAGAGACGAATAAGAAGGA
TGAAGTAGAGAAAGATGAGGCCATGGAGGAGAAAGGAGAAGAAATTGATGGAGAAAAAGT
TAAATCGCCGGTGACTCCAGTCAGTGAGAGACCTATAAGGGAGAGGAAGAGGACTGGCCG
TTACGTTATAGATACTCCTCCTCGATCCTCCGGGAACAAGCCATGTGATCAGCAATTGGG
GATTCAGTTACTCATTAATATGATACGTCAATTGATATATGGAGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGAACAATTATT
   
Sequence Name 38-K111268-0022-288-B07C-8409
GenBank Accession LN503024 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K111268-0022-288-B07C-8409
CGTACATATTAATGAGTAACTGAATCCCCAATTGCTGATCACATGGGCTTGTTCCCGGAG
GATCGAGGAGGAGTATCTATAACGTAACGGCCAGTCCTCTTCCTCTCCCTTATAGGTCTC
TCACTGACTGGAGTCACCGGCGATTTAACTTTTTCTCCATCAATTTCTTCTCCTTTCTCC
TCCATGGCCTCATCTTTCTCTACTTCATCCTTCTTCTTCGTCTCTGTGTCCTTCTCGGTA
ACAGCAACAGTATCTGCTAGTTGTGGTGTTTTCAATTCTAGGGTTTCAGTCGCCATGTGG
AGACGACGACTAACTGAAACAGTGGTGAATAAAGGAGAAAGCTGATACTGTTGCTGT

Last Updated on 06.12.2018 16:11