Sequences from confirmation amplicon


Confirmation sequences for line 288C06 and hit At5g62130 are shown.
Line ID 288C06
Locus At5g62130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24952651
Primers used for PCR 18sa - 8474, show primer details
   
Sequence Name 52-K116229-0022-288-C06C-18sa
GenBank Accession LN507924 [GenBank]
Sequencing primer gene specific primer, 18sa: 5'-TTCTTCACTTCCTCCTTCTCTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116229-0022-288-C06C-18sa
TCTCTTCTTCTCTCTCTCTCTGGCTCTATGCATCCACCTGTCTCCGTAATTTGATGGACA
AGAAGAAACTGCCACACCCTAACCCATTCAATTCCTCCGATCACCCACCGACTTACTCTG
CTCTCTAGAAATTTCCCTACGAGAAAGCTTCTTCCTTTTGTCTTTTTGCTTCTCCTTTCT
TCTTCTCTTCTATTTCTTCTCTCTACTCTCTACTTTCTTCTGTTTTTGGTAATCGGATGG
CGCGAAATCTGCCATGGGTTCTGCTTATTATTGTGGTATCGTGTCTTGTTTCAACACTTG
AAGCTAGTGAAGGAGATTCTGATTCTCTTTACAAGTAAAAAGTCTTCTCCTTTCTCCTTT
TCTAAGATCGCTTCTTTCAATTCACGGCTCTTCTCTGCTTTATTCATATCTGTGGAGAAG
TAAAAATACAAACTTTATTTCTTAATTAGCTTTCTGTTATTGCTTACTGACATTATAAAT
GCTGAGCTATGTTTTGATTTAACTGTCAGAGTCGACCGTTAGATTCATTGATTGCATAAC
TCTGCAAGGATCATTGCTGATCCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAG
   
Sequence Name 12-K116228-0022-288-C06C-8409
GenBank Accession LN491738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116228-0022-288-C06C-8409
AGTTAAATTCAAAACATAGCTTCAGCATTTATAATGTCAGTAAGCAATAACAGAAAGCTA
ATTAAGAAATAAAGTTTGTATTTTTACTTTCTCCAAAGATATGAATAAAGCAGAGAAGAG
CCGTGAATTTGAAAGAAGCGATCTTAGAAAAGGAGAAAGGAGAAGACTTTTTACTTTGTA
AAGAGAATCAGAATCTCCTTCACTAGCTTCAAGTGTTGAAACAAGACACGATACCACAAT
AATAAGCAGAACCCATGGCAGATTTCGCGCCATCCGATTACCAAAAACAGAAGAAAGTAG
AGAGTAGAGAGAAGAAATAGAAGAGAAGAAGAAAGGAGAAGCAAAAAGACAAAAGGAAGA
AGCTTTCTCGTAGGGAAATTTCTAGAGAGCAGAGTAAGTCGGTGGGTGATCGGAGGAATT
GAATGGGTTAGGGTGTGGCAGTTTCTTCTTGTCCATCANATTACGGAGACAGGTGGATGC
ATAGAGCCAGAGAGAGAGAGAAGAAGAGAGAGATCTCGCTGCGGCAAAAGAGAGAGAGAG
AGAGAGAGAGAAATCCCGTGAGGGTGAGAG

Last Updated on 06.12.2018 16:11