Sequences from confirmation amplicon


Confirmation sequences for line 288D01 and hit At4g22180 are shown.
Line ID 288D01
Locus At4g22180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11739345
Primers used for PCR CQ36 - 8474, show primer details
   
Sequence Name 04-K105950-0022-288-D01C-CQ36
GenBank Accession LN487923 [GenBank]
Sequencing primer gene specific primer, CQ36: 5'-AATCTACCTCCTTTCGAGTCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K105950-0022-288-D01C-CQ36
GATTGGTTTCACTCTACACTCCATTACAACGGTAAGGAGTACCACAAACGTATTAGAATA
CTATCCACTGTGTTTTGGATCGATGAGAAAACAAAAGATTACGTAGTTATATGGGGACTT
GGAAGTTCATGTGTGGTTTATTCCAAGAAAGGAGATAAATGTTGGAATCAAATTCCAGAA
ACTTCAAATTGTCATCACATGGTTTACAAGGATCACAAGCTTTACTTCTCTACGTCTACC
TACAAATATGAATTCAGAATCTTTGATTTCTCTAGAGAGATTCCTCAACAAATCTTTCAA
GATAGAAACAGCAATGTATTCAATTGACATGTCCTGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCG
   
Sequence Name 20-K105951-0022-288-D01C-OriL
GenBank Accession LN495216 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K105951-0022-288-D01C-OriL
TCATTGAATACATTGCTGTTTCTATCTTGAAAGATTTGTTGAGGAATCTCTCTAGAGAAA
TCAAAGATTCTGAATTCATATTTGTAGGTAGACGTAGAGAAGTAAAGCTTGTGATCCTTG
TAAACCATGTGATGACAATTTGAAGTTTCTGGAATTTGATTCCAACATTTATCTCCTTTC
TTGGAATAAACCACACATGAACTTCCAAGTCCCCATATAACTACGTAATCTTTTGTTTTC
TCATCGATCCAAAACACAGTGGATAGTATTCTAATACGTTTGTGGTACTCCTTACCGTTG
TAATGGAGTGTAGAGTGAAACCAATCATATATGGTTCGTTCAATCTTTACCATGCCAAGT
TGTGACTCGAAAGAGGGGTAGAT
Primers used for PCR CQ37 - 8474, show primer details
   
Sequence Name 03-K105950-0022-288-D01C-CQ37
GenBank Accession LN487492 [GenBank]
Sequencing primer gene specific primer, CQ37: 5'-TAAAACACCAAACACGTCATCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105950-0022-288-D01C-CQ37
TACATATGAAGAAGAAGTTGAAATCATCAAGTTTGTGTGAACTTGGTAAGAACCATCGAG
CACTAGAGAGTTGAGCTGATGAACAATCAAACTTGTGCACTGGATCCATCTTCTGTGTCT
CGAGACTGAAGATAAATGTATCGTTAATGACCTTCGACTTTGTATGAGTACCACTGAAAT
AAATGGAATTTCCTAGGAGTCCCACAGTGTCATTGGCGAGCATAGTGATACCTAGATCCA
AAAGAATTGCCTCGTCCCCCAAAGATTCAACTTTTTCATATTTCTTATAGGACCCCGATG
AATAAACCTTGTAGACGTAAAAAAATCTGCAGATTCTAGTCTCTTTCTCCACTATTCTGT
CAACCTTAAGGACATCTCCAGTTACTGTGACAACAAGTTTTGTATCAATAGTAGCAAAGG
GATAACCTGGTTGACCACGATGACGATTTAATGTAAGTCCGCGAAGAAATGAGTATGAAA
CCGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 19-K105951-0022-288-D01C-8409
GenBank Accession LN494764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K105951-0022-288-D01C-8409
GTTCTCTCATTTCTTCGCGGACTTACATTAAATCGTCATCGTGGTCAACCAGGTTATCCC
TTTGCTACTATTGATACAAAACTTGTTGTCACAGTAACTGGAGATGTCCTTAAGGTTGAC
AGAATAGTGGAGAAAGAGACTAGAATCTGCAGATTTTTTTACGTCTACAAGGTTTATTCA
TCGGGGTCCTATAAGAAATATGAAAAAGTTGAATCTTTGGGGGACGAGGCAATTCTTTTG
GATCTAGGTATCACTATGCTCGCCAATGACACTGTGGGACTCCTAGGAAATTCCATTTAT
TTCAGTGGTACTCATACAAAGTCGAAGGTCATTAACGATACATTTATCTTCAGTCTCGAG
ACACAGAAGATGGATCCAGTGCACAAGTTTGATTGTTCATCAGCTCAACTCTCTAGTGCT
CGATGGTTCTTACCAAGTTCACACAAACTTGATTGATTTCAACTTTCTTCTTCATATGTT
ATTTTTCTGATTTTCTCTAATGAATAATGATGACGTGTTGGGTGGGTTTTA

Last Updated on 06.12.2018 16:11