Sequences from confirmation amplicon


Confirmation sequences for line 289E11 and hit At5g25430 are shown.
Line ID 289E11
Locus At5g25430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8853951
Primers used for PCR 01yi - 8474, show primer details
   
Sequence Name 29-K120044-0022-289-E11C-01yi
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01yi: 5'-TAATCAAATGAAGGGATGTCGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K120044-0022-289-E11C-01yi
TACTTAAAATGTTAACTGGTTTAGGAAAAGACCTCACAATCTCAGCAAAGCGAGAAATAA
AAAATAAAAAGTACAACATTGTGGAGATGATCGATCCAGATTTGAAGAATAGCTATCCAC
TTGAAGCGGGAAGACTTATGTGCGAGCTTATTAAACAATGCCTAGAGGTAGACCCGAAGA
TGCGACCAACCATGCAAGAAGTGCTAGATAATCTGAATGCTATAGCTCAGATTTGAGGAG
AAACTATATAAGGGTTACTAATGCTGAAAACATTGTTTCACTATAAGACAGTTATAACAT
AGTCAATGTATGCATTTGTATACAATAACTCGTATATGACGAGAAGTATTAAGAAACAAG
ACCTAATTCTCCATTCTTATCCATCACTCAAGAACAGAGCTATATGCTAATCAACTTTGA
ACCTTTACACTATACAATGATCATTATCTTCTTCGACCTCTCTCTCTGCAATACGTTGAA
AATATGATTACTTTGGAGCCACGCTATGTGTCAGAAGAATAACCGAGAGATGACGTGTAA
AGCAAAAAGCGTTGTGAAAATCTTTTATCTCCTCTAAAATAACTTTGACTGGTGTACCAT
TGTTATTCTTGCTAGCTATTAACTGGTCTTGAAATTTGGCGCATTTTTCTTGCAAAGCAA
GCAATACGAGGTGACAAGGTATTCATGGCAGAGGTGATCGATCCGGATTTAGAGAATAGC
TATTAGCTATCCTCATGAAGCGGAAGACATATATATAAGCTTATAAATAATGCTTTCACC
GTCACCCGAATAAATGGCCATAATGCAGNCAGTACTGGATAATTTGAATGCTGAATAATC
TCATATTTAATTG
   
Sequence Name 14-K120043-0022-289-E11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K120043-0022-289-E11C-8409
CATTTCATTGAATTATCCTGAGGTGTACACGTGGGGATGTCTGATTCAGGGGCTTTTTGT
GTACACGTGTGTGATACCTTTAAACCATTTTGTGAGCAGAAACTAGGGTCTTCTCATCAT
TTATCAGAAGGTGAAGATGAATTCTACGACGCGGAGATATTGGATGAGATGACTACAAGC
AGAGGTGAAATAAGGATCCGAACCATAAGTTTTAAAGAGGTAATAAAAACAAATTATATC
TATTCTACACAATCCAAGCATTGATATGAAAAAATGCATATAAACAAGAATCTTGTTTCA
GGTGCACCCGGAGCCTGAAGAGAAGCATGTAACCTTTGAACCACATTAAAAAGAATCTTT
TGCAATTGTTTTTGTACACAACTGTATTTGTGCATAGGCATACATTGACTATGTTATAAC
TTTCCTTATAGTGGAACTTTGTTTCAGCAATTAAATATGAGATTATTCAGCATTCAAATT
ATCCAGTACTTGCTGCATTATGGCCATTTATTCGGGTGACGGTGAAAGCATTATTTATAA
GCTTATATATATGTCTTCCGCTTCATGAGGATAGCTAATAGCTATTCTCTAAATCCGGAT
CGATCACCTCTGCCATGAATACCTTGTCACCTCGTATTGCTTGCTTTGCAAGAAAAATGC
GCCAAATTTCAAGACCAGTTAATAGCTAGCAAGAATAACAATGGTACACCAGTCAAAGTT
ATTTTAGAGGAGATAAAAGATTTTCACAACGCTTTTTGCTTTACACGTCATCTCTCGGTT
ATTCTTCTGACACATAGCGTGGCTCCAAAGTAATCATATTTTCAACGTATTGCAGAGAGA
GAGGTCGAAGAAGATAATGATCATTGTATAGTGTAAAGGTTCAAAGTTGATTAGCATATA
GCTCTGGTCTTGAGTGATGGATAAGAATGGAGAATTAGGTCTTGTTTCTTAAACTTCTCG
TCATA

Last Updated on 06.12.2018 16:11