Sequences from confirmation amplicon


Confirmation sequences for line 289H07 and hit At3g06470 are shown.
Line ID 289H07
Locus At3g06470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1984776
Primers used for PCR 15qb - 8474, show primer details
   
Sequence Name 42-K115305-0022-289-H07C-15qb
GenBank Accession LN504578 [GenBank]
Sequencing primer gene specific primer, 15qb: 5'-GCATTTAGAGATGATAAATTTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115305-0022-289-H07C-15qb
ATTCTTGCTTACTCATTGCATGAATTCTTTAGATTGAACGTACTTTTCAGTCAAAGAATT
AGAGATGATGAGTCCATGTCATGACTTTTGTGATATTTTGATATTTTCTGTCATCTTATA
TTCATACAAGTACAAAAGTATCTCAACATTTGCAAGTACAACACAGACAAAAATTTCAAA
ATAAACAACAATTCAAGACAATTAATCCTTTTGTGTGGAAACTAATCAACAATTCAAGAC
ACAATGAGAGGTTTTACTTATTGTCCAAGAAACCAATCTAATCGCTTTTTTTGCCATCCT
CTCTCGTTGGCTTCTTGACATAATTCTTTGAATGGAAGTTGGAAAAGAGAGCCAAAAGAG
AAGCATTAAATGCAGCGTTGAAACACCATCCCCAAATCCCGGTGCAACCCGACCCGAATA
AGTGCTCTCGGAGCATCCAACCGGATAACCCGAAACTGAAAACAAACTGAACAATCTGAC
AATCCGTCACCAATCTCTTCCACTTGGGCCTCGATCCAACGGCGCAAAGGAAGTAGTAAC
CGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCG
   
Sequence Name 07-K115304-0022-289-H07C-8409
GenBank Accession LN489401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115304-0022-289-H07C-8409
ATTTACATTGATATACGGTTACTACTTCCTCTGCGCCGTTGGATCGAGGCCCAAGTGGAA
GAGATTGGTGACGGATTGTCAGATTGTTCAGTTTGTTTTCAGTTTCGGGTTATCCGGTTG
GATGCTCCGAGAGCACTTATTCGGGTCGGGTTGCACCGGGATTTGGGGATGGTGTTTCAA
CGCTGCATTTAATGCTTCTCTTTTGGCTCTCTTTTCCAACTTCCATTCAAAGAATTATGT
CAAGAAGCCAACGAGAGAGGATGGCAAAAAAAGCGATTAGATTGGTTTCTTGGACAATAA
GTAAAACCTCTCATTGTGTCTTGAATTGTTGATTAGTTTCCACACAAAAGGATTAATTGT
CTTGAATTGTTGTTTATTTTGAAATTTTTGTCTGTGTTGTACTTGCAAATGTTGAGATAC
TTTTGTACTTGTATGAATATAAGATGACAGAAAATATCAAAATATCACAAAAGTCATGAC
ATGGACTCATCATCTCTAATTCTTTGACTGAAAAGTACGTTCAATCTAAAGAATTCATGC
AATGATGTAAGCAAGAATTGAATTTAAACATCGTTCACCATTCCAAGAACATTGTAAATT
TATCAT

Last Updated on 06.12.2018 16:11