Sequences from confirmation amplicon


Confirmation sequences for line 290A03 and hit At2g44180 are shown.
Line ID 290A03
Locus At2g44180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18269502
Primers used for PCR 44fr - 8474, show primer details
   
Sequence Name 21-K111563-0022-290-A03C-44fr
GenBank Accession LN495720 [GenBank]
Sequencing primer gene specific primer, 44fr: 5'-TGGATCAACAGGTAACAACAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K111563-0022-290-A03C-44fr
GAATGTGGATTTTACGAATTCGAGTAAATTGGTTTTTCACTTGTGTCATTTTGCAGGGAA
AGGATATGTAAGAGAAGACTTAGAATGTAGCCATTACATGAAAAACTATGATGTGGGTCA
TGTACCTTTGAGGTTACCAAGGGCAAAACAACTCCTTGCCACTATTAACAAGAACTTCTC
CACGCTAGCCTTCTGCAGACGTTATTTGGACCGCCTTGGTGAAACAAAATACTTGATGGC
TTTAAAGAATCTTTGTGATTCTGGCATAATTGAGGTAAACTCCTTAAAACTATATATATA
CTCTGATGACTTATGTTCAAGAACATTTTGATGAAAGTAAGTTTCTTTGGGTTAACTTTT
GTAGCCATGTCCTCCAGTGTGTGATGTCAAAGGAAGCTATATTTCTCAGTTTGAGTATGA
TGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTATT
   
Sequence Name 05-K111556-0022-290-A03C-8409
GenBank Accession LN488417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K111556-0022-290-A03C-8409
ATTTACTGAAGCCTCATACTCAAACTGAGAAATATAGCTTCCTTTGACATCACACACTGG
AGGACATGGCTACAAAAGTTAACCCAAAGAAACTTACTTTCATCAAAATGTTCTTGAACA
TAAGTCATCAGAGTATATATATAGTTTTAAGGAGTTTACCTCAATTATGCCAGAATCACA
AAGATTCTTTAAAGCCATCAAGTATTTTGTTTCACCAAGGCGGTCCAAATAACGTCTGCA
GAAGGCTAGCGTGGAGAAGTTCTTGTTAATAGTGGCAAGGAGTTGTTTTGCCCTTGGTAA
CCTCAAAGGTACATGACCCACATCATAGTTTTTCATGTAATGGCTACATTCTAAGTCTTC
TCTTACATATCCTTTCCCTGCAAAATGACACAAGTGAAAAACCAATTTTACTCTGAATTC
GTAAAAATCCACATTTCTTGTATAGTTTCATGTGAGAAATGTTGTTGTTACCTGTTGATC
C

Last Updated on 06.12.2018 16:11