Sequences from confirmation amplicon


Confirmation sequences for line 290B03 and hit At3g03220 are shown.
Line ID 290B03
Locus At3g03220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:742953
Primers used for PCR KD79 - 8474, show primer details
   
Sequence Name 60-K103246-0022-290-B03C-KD79
GenBank Accession LN510232 [GenBank]
Sequencing primer gene specific primer, KD79: 5'-TGGTCATTGTAATCCACCTAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103246-0022-290-B03C-KD79
GTTGAGAGATCGCTATTTGGAAGCTGGGAACATGCCGGTGCAGTATCGAAGGTGCGTTTC
TCTCCGCTTTTTCCCAATTTGTTTCGCGTTTTGTTAAAAAAATGCGTTTGCGGTGGTGGG
GAACGCGGTTCTGCTTTTAGACGCCACTATCGATTTAAAAAATCCGGCGATGCTTTTGCT
TTTGTGTCTTCTTCGATATCCGTGTGCTTTATTATGTGAGATGGGTTTACCCTAAATCTC
TTCCTCAATATGCAATTTACTTTATTGCCCCTAATCGTTTTATCTTAGTGTGTACAAGAT
TCTGAGAAATGGTAGATAGGTTTGCTCATCATAGTTGAGTAGCTTTGCTCTCATTTTCAT
CTTAGCCATGAGAGGACTGAAATTGACTGTTTCTGTGAAGTTCTAGGCACAATGCGGTAA
ACTACTATNTAGGTTCATGATACAATGGATCATTAGATCCTTTATTAGTTTGTGAAGTGT
TTTGGAGCTGAGCCTATNAATNGTTAGTTGCATATCANTTAAGTTATGAGTTATGACTTA
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATTTCCGCA
   
Sequence Name 12-K103247-0022-290-B03C-8409
GenBank Accession LN491535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103247-0022-290-B03C-8409
TAGTCATAACTCTAACTTAAGTGATATGCAACTAACTATTTATAGGCTCAGCTCCAAAAC
ACTTCACAAACTAATAAAGGATCTAATGATCCATTGTATCATGAACCTAAATAGTAGTTT
ACCGCATTGTGCCTAGAACTTCACAGAAACAGTCAATTTCAGTCCTCTCATGGCTAAGAT
GAAAATGAGAGCAAAGCTACTCAACTATGATGAGCAAACCTATCTACCATTTCTCAGAAT
CTTGTACACACTAAGATAAAACGATTAGGGGCAATAAAGTAAATTGCATATTGAGGAAGA
GATTTAGGGTAAACCCATCTCACATAATAAAGCACACGGATATCGAAGAAGACACAAAAG
CAAAAGCATCGCCGGATTTTTTAAATCGATAGTGGCGTCTAAAAGCAGAACCGCGTTCCC
CACCACCGCAAACGCATTTTTTTAACAAAACGCGAAACAAATTGGGAAAAAGCGGAGAGA
AACGCACCTTCGATACTGCACCGGCTTGTTCCCAGCTTTCCAAATAGCGATCTTCTCAAA
CGCTTCGATCGGAAGCACGAAATGTTTGTTAGGTGGATTACAAATGA
Primers used for PCR KD80 - 8474, show primer details
   
Sequence Name 09-K103729-0022-290-B03C-KD80
GenBank Accession LN490144 [GenBank]
Sequencing primer gene specific primer, KD80: 5'-TCATGGGATTGTATCTCAATGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103729-0022-290-B03C-KD80
GGTCTGTCCTAATTCCAGTTTTTCGGGGAGACATTGTAAGATGTCACGGTTGACCTGTCA
CTAGAAGTTACTTCAAATGAGAGAGCTTGGTTCCTGAGATCAGCATTAATATGCCAATTT
TGTCCCCAATTACGACCCATAGGTAACCACCCGGTTCTCGACCCTTTGATCTTCACAGCA
GCTATATCACCGGACCCTGCAACATTGGTGATCAAAACCGAAATGAAAATGCCTCCACCA
TCGACTGTAAACCGCATGCTCCCTTCCTTTCTACATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K103730-0022-290-B03C-8409
GenBank Accession LN497407 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103730-0022-290-B03C-8409
AGGAAGGGAGCATGCGGTTTACAGTCGATGGTGGAGGCATTTTCATTTCGGTTTTGATCA
CCAATGTTGCAGGGTCCGGTGATATAGCTGCTGTGAAGATCAAAGGGTCGAGAACCGGGT
GGTTACCTATGGGTCGTAATTGGGGACAAAATTGGCATATTAATGCTGATCTCAGGAACC
AAGCTCTCTCATTTGAAGTAACTTCTAGTGACAGGTCAACCGTGACATCTTACAATGTCT
CCCCGAAAAACTGGAATTATGGACAGACCTTCGAAGGGAAACAATTCGAGACTCCGTGAG
ACAGAGACGGACACATTGAGATACAATCCCATG

Last Updated on 06.12.2018 16:11