Sequences from confirmation amplicon


Confirmation sequences for line 290B04 and hit At3g09540 are shown.
Line ID 290B04
Locus At3g09540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2929491
Primers used for PCR CL82 - 8474, show primer details
   
Sequence Name 53-K032927-022-290-B04C-8409
GenBank Accession LN508058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K032927-022-290-B04C-8409
TTACATTGAATAATCCTGTTGTTTTGATAGATACATTCTCAGTGCAACATATATGAAGCT
GGTGAAAAGAAAACTGTCTTTAAGTACATCACTGAGAAGGTACGTAATATCCTTTGTACG
TTCTTGAGGTTCTTAAATCATTTTCTTTATAATGTTATAGTTAACATTACGTTTTTTTGT
GTTTGGTGATTAGGCCGCTGATAAAGAGAAACCCGGAGCTGGATTCGTAAGATCAGAAGG
GGATTTGTTGTTGAACGGAGCCAAATCGTGTCTTAGTCAGGGCGGAGAGCGCTATGTGTT
CTCTCCAATCCAACACTATTCTGAATGGACCGTCGAATCTCCAACCGATATACTCAAGAA
CTATCTGAAACACTCGACCGGCTGGCAGAACCTTCCCCTCCCACTAGACCGGCCTCCGAC
CACCGCATAACTATTGCTTAGCGAAGTGAATTGATTGGATCACTTTTGGCACTACCAAAA
CTATATTGCAGTAGTTTCTTCCATTTCCACTTAGACAAACAAAATGATATATCAAATTTG
ATAGGATTGTCAAAATTTTTC
   
Sequence Name 53-K032927-022-290-B04C-CL82
GenBank Accession LN508059 [GenBank]
Sequencing primer gene specific primer, CL82: 5'-GGAAAAATTCGACAATCCTATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K032927-022-290-B04C-CL82
GTGGAATGGAAGAACTACTGCAATATAGTTTTGGTAGTGCCAAAAGTGATCCAATCAATT
CACTTCGCTAAGCAATAGTTATGCGGTGGTCGGAGGCCGGTCTAGTGGGAGGGGAAGGTT
CTGCCAGCCGGTCGAGTGTTTCAGATAGTTCTTGAGTATATCGGTTGGAGATTCGACGGT
CCATTCAGAATAGTGTTGGATTGGAGAGAACACATAGCGCTCTCCGCCCTGACTAAGACA
CGATTTGGCTCCGTTCAACAACAAATCCCCTTCTGATCTTACGAATCCAGCTCCGGGTTT
CTCTTTATCAGCGGCCTAATCACCAAACACAAAAAAACGTAATGTTAACTATAACATTAT
AAAGAAAATGATTTAAGAACCTCAAGAACGTACAAAGGATATTACGTACCTTCTCAGTGA
TGTACTTAAAGACAGTTTTCTTTTCACCAGCTTCATATATGTTGCACTGAGAATGTATCT
ATCAAAACAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11