Sequences from confirmation amplicon


Confirmation sequences for line 290D04 and hit At1g14480 are shown.
Line ID 290D04
Locus At1g14480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4957205
Primers used for PCR FV85 - 8474, show primer details
   
Sequence Name 13-K100894-0022-290-D04C-8409
GenBank Accession LN491958 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K100894-0022-290-D04C-8409
GAGCCTTTACAATTGAATATTGATGATTACATTCGTTAATATTGATGATTACATTCGTTG
ATATTGATGATTACATTGTTTTTGAATATTTTTAGCCATAATCACCATTATATAGTTGTA
ACACGAAACGTCGTTAATATTGATGATTTAACATTCGTTGATATCTTAAGGACCCAGGGA
GAGAACGCAGGTGGTGGGAACCTAGATTTGGAACAAGCTGTTATAAAAACTGGGTGTGTA
GAGGCGGCTTCTATGCCAAAATTCAAGGAAGAGTCTGATTTGTTGAAATCACCAATCAAT
TTTATGACATACTATTCCACGTCCATGAAACGCATGAAGTCTTCCACTTCAGATCAAGAT
CGCGGAGCATTTCTGATTGTATGCACTTTGATAATAACAGCCACTTATCAGATGGCCCTC
CAGCCTCCCGGAGGTGTACACCAATCTGAGAATGCGAATGCGAATGCGGGTTCTGTGGTG
ATGAAACAGACATTCTTCATCCTTCTTTGGATTTCCAACACCGTTGGTTTCTGCTGCGCT
GTATTCTACACCTTTTGTCTCATACCACTCGGTCAGTTGTTTACGATATGGTTTTTTTAT
ATAGGGACATGTCTGTGCATTTCTTATGCACTAGCAATGGCAGTAATCTCACCACACCCT
CTAG
   
Sequence Name 13-K100902-0022-290-D04C-FV85
GenBank Accession LN491959 [GenBank]
Sequencing primer gene specific primer, FV85: 5'-AGCAGAGAGAAACACTAGAGGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K100902-0022-290-D04C-FV85
GAATGCACAGACATGTCCCTATATAAAAAACCATATCGTAAACAACTGACCGAGTGGTAT
GAGACAAAAGGTGTAGAATACAGCGCAGCAGAAACCAACGGTGTTGGAAATCCAAAGAAG
GATGAAGAATGTCTGTTTCATCACCACAGAACCCGCATTCGCATTCGCATTCTCAGATTG
GTGTACACCTCCGGGAGGCTGGAGGGCCATCTGATAAGTGGCTGTTATTATCAAAGTGCA
TACAATCAGAAATGCTCCGCGATCTTGATCTGAAGTGGAAGACTTCATGCGTTTCATGGA
CGTGGAATAGTATGTCATAAAATTGATTGGTGATTTCAACAAATCAGACTCTTCCTTGAA
TTTTGGCATAGAAGCCGCCTCTACACACCCNGTTTTTATAACAGCTTGTTCCAAATCTAG
GTTCCCACCACCTGCGTTCTCTCCCTGGGTCCTTAAGATATCAACGAATGTTAAATCATC
AATATTAACGACGTTTCGTGTTACAACTATNTNATGGTGATTATGGCTAANAATATTCAA
AAACAATGTAATCATCAATATCAACGAATGTAATCATCAATATNAACGAATGTAATCATC
AATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGTAG
ATT

Last Updated on 06.12.2018 16:11