Sequences from confirmation amplicon


Confirmation sequences for line 290D05 and hit At5g44670 are shown.
Line ID 290D05
Locus At5g44670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18020639
Primers used for PCR 23fb - 8474, show primer details
   
Sequence Name 55-K111269-0022-290-D05C-23fb
GenBank Accession LN508848 [GenBank]
Sequencing primer gene specific primer, 23fb: 5'-TCTGCACTTTAGCTACTCTCCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K111269-0022-290-D05C-23fb
TCGCTTCGACTCCGTTTCTGCATCTCACGCATCGCCGTAAACTCCACCTCCGTCAACTTC
ACCACCGTCGTCGAAAAGCCAGTCTTAGATAACGCTGTCAAGTTAACTGAGAAGCCGGTG
TTGGATAACGGCGTTACGAAACAGCCGTTAACTGAAGAGAAGGTGTTAAATAACGGCGTT
ATTAAACGGACGTTTACTGGTTACGGCTGGGCAGCTTATAACTTCGTGTTAATGAACGCT
TACAGAGGCGGCGTTAACACATTCGCCGTTATCGGTTTATCATCTAAACCACTTCACGTT
TACTCTCATCCCACTTACCGTTGCGAATGGATTCCACTAAACCAATCCGATAACCGGATT
TTAACCGACGGTACCAAAATCTTAACCGATTGGGGTTACGGTAGAGTTTACACAACCGTC
GTCGTAAACTGTACTTTTCCGTCAAACACCGTGATAAACCCTAAAAACA
   
Sequence Name 39-K111268-0022-290-D05C-8409
GenBank Accession LN503392 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K111268-0022-290-D05C-8409
TTTCATTGAATATCATGTCGATTTATAAGGGCAATTGTTTTTAGGGTTTATCACGGTGTT
TGACGGAAAAGTACAGTTTACGACGACGGTTGTGTAAACTCTACCGTAACCCCAATCGGT
TAAGATTTTGGTACCGTCGGTTAAAATCCGGTTATCGGATTGGTTTAGTGGAATCCATTC
GCAACGGTAAGTGGGATGAGAGTAAACGTGAAGTGGTTTAGATGATAAACCGATAACGGC
GAATGTGTTAACGCCGCCTCTGTAAGCGTTCATTAACACGAAGTTATAAGCTGCCCAGCC
GTAACCAGTAAACGTCCGTTTAATAACGCCGTTATTTAACACCTTCTCTTCAGTTAACGG
CTGTTTCGTAACGCCGTTATCCAACACCGGCTTCTCAGTTAACTTGACAGCGTTATCTAA
GACTGGCTTTTCGACGACGGTGGTGAAGTTGACGGAGGTGGAGTTTACGGCGATGCGTGA
GATGCAGAAACGGAGTTCGGAAGCGGAGATGGAGAAAGAAGAAGGTAGAAAAGGGAGGAG
AGTAGCTAAAGTGCAGA

Last Updated on 06.12.2018 16:11