Sequences from confirmation amplicon


Confirmation sequences for line 290D07 and hit At1g02840 are shown.
Line ID 290D07
Locus At1g02840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:627919
Primers used for PCR PM02 - 8474, show primer details
   
Sequence Name 27-K021852-022-290-D07C-8409
GenBank Accession LN498194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K021852-022-290-D07C-8409
TGAGCCATTTACAATTGAATATATTGGACAATCCATCGAAAACCAAGTTGAATGGTTTTA
ATCTTGGACAATCTTACTGTAGGGACAACTGGAGTTGTTGATTACACCTGCTATGAGGAC
ATGAAGTATGCGGTAAGCTATTTTCCCTGTTTCTCTTCTGTGGTTGAGAGTTTGTTCTAC
TCATCCTTAATCACTGATTAGCTCTCACTCTTGAATTTCCCCAGCTGAAAAAGCTCGACG
ACACAGAGTTTCGAAATGCGTTTTCGAATGGATATGTCCGGGTTAGTTGAAGCTGACTTT
CCTGTGAATTGTGATCTTTTCATTGTTGTGTTTAATATTTTGTTTCATATCTCTTTAGGT
TAGAGAATATGATTCAAGGAAGGATTCTAGGAGTCCTAGCCGGGGAAGATCCTATTCTAA
GAGCCGCAGCCGCAGCCGTGGACGAAGCGTGAGCCGAAGCAGGAGCAGAAGCAGGAGCAG
GAGCAGAAGTCCCAAGGCAAAGTCTTCACGTAGGTCCCCTGCAAAATCTACATCAAGATC
TCCTGGCCCCCGCTCGAAGTCAAGGTCACCGTCTCCAAGAAGGTAATGATTTGATTCCTT
TTCAGAATGCACCAGGCAACATTACTTAGTCGGTAGATTTT
   
Sequence Name 28-K021852-022-290-D07C-PM02
GenBank Accession LN498664 [GenBank]
Sequencing primer gene specific primer, PM02: 5'-AACTTCACAGGAAAATCTACCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K021852-022-290-D07C-PM02
GATCAAATCATTACCTTCTTGGAGACGGTGACCTTGACTTCGAGCGGGGGCCAGGAGATC
TTGATGTAGATTTTGCAGGGGACCTACGTGAAGACTTTGCCTTGGGACTTCTGCTCCTGC
TCCTGCTTCTGCTCCTGCTTCGGCTCACGCTTCGTCCACGGCTGCGGCTGCGGCTCTTAG
AATAGGATCTTCCCCGGCTAGGACTCCTAGAATCCTTCCTTGAATCATATTCTCTAACCT
AAAGAGATATGAAACAAAATATTAAACACAACAATGAAAAGATCACAATTCACAGGAAAG
TCAGCTTCAACTAACCCGGACATATCCATTCGAAAACGCATTTCGAAACTCTGTGTCGTC
GAGCTTTTTCAGCTGGGGAAATTCAAGAGTGAGAGCTAATCAGTGATTAAGGATGAGTAG
AACAAACTCTCAACCACAGAAGAGAAACAGGGAAAATAGCTTACCGCATACTTCATGTCC
TCATAGCAGGTGTAATCAACAACTCCAGTTGTCCCTACAGTAAGATTGTCCAAGATTAAA
ACCATTCAACTTGGTTTTCGATGGATTGTCCAATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATAACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT

Last Updated on 06.12.2018 16:11