Sequences from confirmation amplicon


Confirmation sequences for line 290E08 and hit At1g65480 are shown.
Line ID 290E08
Locus At1g65480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24332353
Primers used for PCR KO01 - 8474, show primer details
   
Sequence Name 58-K024138-022-290-E08C-KO01
GenBank Accession LN509587 [GenBank]
Sequencing primer gene specific primer, KO01: 5'-TAAGCTCAATGATATTCCCGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K024138-022-290-E08C-KO01
CACTTTAAAGTGAAAAAAACAAATCTTAGTTCAAGTCACGTCCTATACATTCTATAGAAT
CTATGTGCGATCAGTAAAATACACAGACATACATAATACATATTTACATTACACATAGTA
AAGTTTTTGAACAAAATATGTCAATTTAGCTTGGGTGTGGGCTTTTTTGGGAGACAAATT
GATGCATCGCACTCTCGAAGTTGATTTTATATGTCTCCTTCTATTAAATGTAAAATGTTT
GAGTCTTAAGTTATAAGTGATCTACTCAATTTCAAAGTATCCAAAATACATGTCCAGTTT
TACATGTGTAGTTGAGTGTCTATATATATAATTCGAGCAACATGTTTGAGCTATGCTATA
ACTTAAAAGTGAATTAATTAAAGAGTTGAGTTAGTGCACAAACCAATGGAGATATTCTCG
GAGGTGAGGGTTGCTAGGACTTGGAACATCTGGATCCACCATAACCTTCATTAAACAAAT
AAGACGAAGAATTATAAGAAGATAAGATTATCATTTTCAAAGAAATCAAGAAAGCAAAAG
GTCTTTTTTTCTTTTTCTTTTTTGAAGTTTGGAAAATGAGATAACACAAGAAAGAAGAAG
AAAACTATGAATAGAAAAAAGGTAGTAGCTACATGTCTGGGAAATCTACATGGATCAGCA
ATGAGTATGAATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCCGCAGCGTTATTATAAAATGAAAGT
   
Sequence Name 59-K024138-022-290-E08C-8409
GenBank Accession LN509876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K024138-022-290-E08C-8409
GTAGATTTCCCAGACATGTAGCTACTACCTTTTTTCTATTCATAGTTTTCTTCTTCTTTC
TTGTGTTATCTCATTTTCCAAACTTCAAAAAAGAAAAAGAAAAAAAGACCTTTTGCTTTC
TTGATTTCTTTGAAAATGATAATCTTATCTTCTTATAATTCTTCGTCTTATTTGTTTAAT
GAAGGTTATGGTGGATCCAGATGTTCCAAGTCCTAGCAACCCTCACCTCCGAGAATATCT
CCATTGGTTTGTGCACTAACTCAACTCTTTAATTAATTCACTTTTAAGTTATAGCATAGC
TCAAACATGTTGCTCGAATTATATATATAGACACTCAACTACACATGTAAAACTGGACAT
GTATTTTGGATACTTTGAAATTGAGTAGATCACTTATAACTTAAGACTCAAACATTTTAC
ATTTAATAGAAGGAGACATATAAAATCAACTTCGAGAGTGCGATGCATCAATTTGTCTCC
CAAAAAAGCCCACACCCAAGCTAAATTGACATATTTTGTTCAAAAACTTTACTATGTGTA
ATGTAAATATGTATTATGTATGTCTGTGTATTTTACTGATCGCACATAGATTCTATAGAA
TGTATAGGACGTGACTTGAACTAAGATTTGTTTTTTTCACTTTAAAGTGGTCTTTGTACG
GGAAT

Last Updated on 06.12.2018 16:11