Genotyping and primer details


Genotyping and primer details for 290E08 - At1g65480
Line ID 290E08
Locus At1g65480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24332353 of line 290E08 Chr1:24332353
   
Insertion allele amplicon 8474 - KO01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KO01: 5'-TAAGCTCAATGATATTCCCGTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24333019
   
wt amplicon (over InsPos) AG12 - KO01
wt amplicon start Chr1:24332081
wt amplicon end Chr1:24333019
wt amplicon size 939 bp
Locus specific primer KO01: 5'-TAAGCTCAATGATATTCCCGTACA-3'
2nd locus specific primer AG12: 5'-GGATTTTTCTTTGTTCCTCCTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11