Sequences from confirmation amplicon


Confirmation sequences for line 291A12 and hit At1g68110 are shown.
Line ID 291A12
Locus At1g68110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25524698
Primers used for PCR 22cy - 8474, show primer details
   
Sequence Name 19-K109234-0022-291-A12C-22cy
GenBank Accession LN494794 [GenBank]
Sequencing primer gene specific primer, 22cy: 5'-AAGAACGGATCCATTTGTAGACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K109234-0022-291-A12C-22cy
ATCGTCGTGGGAGGTGGCTTTGATGATAGCTGCTTCGAGATCTGCGTTCCGGTAAGAGCT
GTTTCGCCGTGAGAAACCAACGGCTAGTAAGCTTTTCCGGTCTTTGATTGCGGCGGCAGC
TCGTTTCCACAGCTTCATCGTCCGAGCCGCCTCCCTCCTAATCTGTTATTCCTCTCTCTC
TCTCGCTTCTTCCTCTGTTTTCTTCTTCTTTCTTCTAACCTGGAGAATGATAAAAGTCAT
GTCTGAAAATGTTTTGTCTCTATTTTGAGTTCTTCTTACCTTTACCATCCGTTACTACCG
TTGTAATCGCTCTGTTTTATCATATTCTTTTTGGTATTTTTCATTGGTAATTGTTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCATTCAAAAATGTA
GATGTCCGC
   
Sequence Name 03-K109233-0022-291-A12C-8409
GenBank Accession LN487523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109233-0022-291-A12C-8409
GCCATTTACATTGAATATATCCTGAACATTACCAATGAAAAATACCAAAAAGAATATGAT
AAAACAGAGCGATTACAACGGTAGTAACGGATGGTAAAGGTAAGAAGAACTCAAAATAGA
GACAAAACATTTTCAGACATGACTTTTATCATTCTCCAGGTTAGAAGAAAGAAGAAGAAA
ACAGAGGAAGAAGCGAGAGAGAGAGAGGAATAACAGATTAGGAGGGAGGCGGCTCGGACG
ATGAAGCTGTGGAAACGAGCTGCCGCCGCAATCAAAGACCGGAAAAGCTTACTAGCCGTT
GGTTTCTCACGGCGAAACAGCTCTTACCGGAACGCAGATCTCGAAGCAGCTATCATCAAA
GCCACCTCCCACGACGATTCCTCCGTCGACTATTCCAACGCTCACCGTGTCTACAAATGG
ATCCGTTC

Last Updated on 06.12.2018 16:11