Sequences from confirmation amplicon


Confirmation sequences for line 291A12 and hit At5g62910 are shown.
Line ID 291A12
Locus At5g62910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25250558
Primers used for PCR BG39 - 8474, show primer details
   
Sequence Name 91-K101673-0022-291-A12C-8409
GenBank Accession LN515108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101673-0022-291-A12C-8409
TTTAATCGTTGTTTTCGTTCGATGCTAAATGACTAAAGTACCCTCAACGTACGTGTGGGT
TCTTCTGTAAGTCGGTGAGTGTGCACGTGTGAGGTAAACGATGTCGTATTTTTGAGTCTA
ACTATGGCAAAAGAGATATACTTTTACCTTTTTTTTTTTGTGTTCTGAAAAGATCTTTGA
TGATATTTTTTGGGAGTTTCCTTATATGCAATTTGCAAACTTGTAATCTTGTGAGTGAAA
GCTGTGTAATGATTCTTTCTTCTTCTTCATACAAAGATTTTTTTATGTAACCCAAAAAGA
AAATACTATGGTTTTGTTGGTTTTTTGTTAGGGGCATATAGTTCTTTTATAACAATTTTT
CTTCTTCGGAATTTATGGCAGGTTAGTTATTATTGATATGTTAAGCTACATGAATAATTA
AATAGAGTTGAATAATAAAAACATACAACAAACCAATACGGTTGAATAAAAAGGT
   
Sequence Name 91-K101680-0022-291-A12C-BG39
GenBank Accession LN515109 [GenBank]
Sequencing primer gene specific primer, BG39: 5'-AGCTTTTGCAACCTTTTTATTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101680-0022-291-A12C-BG39
TTTATATTCACTCTATTTAATTATTCATGTAGCTTAACATATCAATAATAACTAACATGC
CATAAATTCCGAAGAAGAAAAATTGTTATAAAAGAACTATATGCACCTAACAAAAAACCA
ACAAAACCATAGTATTTTCTTTTTGGGTTACATAAAAATATCTTTGTATGAAGAAGAAGA
AAGAATCATTACACAGCTTTCACTCACAAGATTACAAGTTTGCAAATTGCATATAAGGAA
ACTCCCAAAAAATATCATCAAAGATCTTTTCAGAACACAAAAAAAAAAAGGTAAAAGTAT
ATCTCTTTTGCCATAGTTAGACTCAAAAATACGACATCGTTTACCTCACACGTGCACACT
CACCGACTTACAGAAAAACCCACACGTACGTTGAGGGTACTTTAGTCATTTAGCATCGAA
CGAAAACAACGATAAATTTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATTTCCGCA

Last Updated on 06.12.2018 16:11