Sequences from confirmation amplicon


Confirmation sequences for line 292E02 and hit At2g45110 are shown.
Line ID 292E02
Locus At2g45110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18599814
Primers used for PCR CQ43 - 8474, show primer details
   
Sequence Name 08-K105950-0022-292-E02C-CQ43
GenBank Accession LN489733 [GenBank]
Sequencing primer gene specific primer, CQ43: 5'-GATTGGAGAGTTTGACCTCCATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K105950-0022-292-E02C-CQ43
CAATATACAATTTCCTTGAAAGTTGAACGCGTCGTGAGAGTGTTCAAAAAATAAACCATT
ATAAAAACTAAAAAGCTTTGTCATCTAATGAATCAAATTCACAAATACCTTTAAAAGAGT
ATAGAAAGCTTGGTTTATACAAAACAAAGTATATAGCTAGATATATCAATGGCATCATCA
CAAAGATATTTCGCTCTCCTAGCTCTTTTCGCAGTCTCACTAAAATTTTGTTATTGCCAA
AATGAGACTATAGATGTAGCTGGATCGGGAACCGCCGGAGTTACTTGGTACGGTGAACCA
TTCGGCGCCGGTAGCACCGGTACGTAATAATATTACATATTTCTAACAATATATATACAT
AAATTCTTAGAATAGTAAATTCATTTATATGATGCTTAAATGTGTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K105951-0022-292-E02C-8409
GenBank Accession LN496995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105951-0022-292-E02C-8409
TTACACACATTTAAGCATCATATAAATGAATTTACTATTCTAAGAATTTATGTATATATA
TTGTTAGAAATATGTAATATTATTACGTACCGGTGCTACCGGCGCCGAATGGTTCACCGT
ACCAAGTAACTCCGGCGGTTCCCGATCCAGCTACATCTATAGTCTCATTTTGGCAATAAC
AAAATTTTAGTGAGACTGCGAAAAGAGCTAGGAGAGCGAAATATCTTTGTGATGATGCCA
TTGATATATCTAGCTATATACTTTGTTTTGTATAAACCAAGCTTTCTATACTCTTTTAAA
GGTATTTGTGAATTTGATTCATTAGATGACAAAGCTTTTTAGTTTTTATAATGGTTTATT
TTTTGAACACTCTCACGACGCGTTCAACTTTCAAGGAAATTGTATATTTGCAATATAAGT
GAATCTTTGTATCTATGGAGGTCAACCCCTCCAAT

Last Updated on 06.12.2018 16:11