Sequences from confirmation amplicon


Confirmation sequences for line 292F01 and hit At5g14720 are shown.
Line ID 292F01
Locus At5g14720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4752281
Primers used for PCR 13dl - 8474, show primer details
   
Sequence Name 26-K110037-0022-292-F01C-13dl
GenBank Accession LN497974 [GenBank]
Sequencing primer gene specific primer, 13dl: 5'-GTGATATGGCATTGATGATGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K110037-0022-292-F01C-13dl
TGCTGGCGAATTGGAAGAACCACATACAGATTGATGCTCTCTTCAGTCGACCTCTTTTGT
AAATTTGTTGAAACTTACGGGGTCGAAGGTGTGTAGCATATATGCTATAAGAAGATTATA
AAGTAAAAATTATGGAATCGGGTTCAGAGAAAAAGTTCCCTCTCAATGCAAAAGACTACA
AGTTATATGAAGAAATTGGAGATGGTGTCAGTGCGACTGTGCATAGAGCTTTGTGTATAC
CGCTTAATGTGGTAGTTGCTATCAAGGTTCTTGATCTGGAAAAGTGCAACAACGATCTGG
TAGGAACTTTTATTCTGAATTATGAAAGAACAACTTCCTTATTCCTAGTCTCTAGCTATT
TCCTGGAGTTACATCAAAGATAGAAGTTTAGTTTTCTCTTACTTTTGTTTTCCTGCTTCA
TGCTTTACTAATAGCGAGTCCAATTGTAGTTTAATTGCCTTTGAAAGTAGAGAACTCCAG
ATCAGTCTGTCAACTGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGGTTAT
   
Sequence Name 10-K110036-0022-292-F01C-8409
GenBank Accession LN490704 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K110036-0022-292-F01C-8409
TGAAGCCATTTACAACAGTTGACAGACTGATCTGGAGTTCTCTACTTTCAAAGGCAATTA
AACTACAATTGGACTCGCTATTAGTAAAGCATGAAGCAGGAAAACAAAAGTAAGAGAAAA
CTAAACTTCTATCTTTGATGTAACTCCAGGAAATAGCTAGAGACTAGGAATAAGGAAGTT
GTTCTTTCATAATTCAGAATAAAAGTTCCTACCAGATCGTTGTTGCACTTTTCCAGATCA
AGAACCTTGATAGCAACTACCACATTAAGCGGTATACACAAAGCTCTATGCACAGTCGCA
CTGACACCATCTCCAATTTCTTCATATAACTTGTAGTCTTTTGCATTGAGAGGGAACTTT
TTCTCTGAACCCGATTCCATAATTTTTACTTTATAATCTTCTTATAGCATATATGCTACA
CACCTTCGACCCCGTAAGTTTCAACAAATTTACAAAAGAGGTCGACTGAAGAGAGCATCA
ATCTGTATGTGGTTTCTTCCAATTCGCCAGCATTTTACTTTTCGTCAACGTCGAGCAAAT
GCGGAAACATCATCAATGGCATATCA

Last Updated on 06.12.2018 16:11