Sequences from confirmation amplicon


Confirmation sequences for line 292G06 and hit At1g80120 are shown.
Line ID 292G06
Locus At1g80120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30139222
Primers used for PCR 16vf - 8474, show primer details
   
Sequence Name 28-K117753-0022-292-G06C-16vf
GenBank Accession LN499024 [GenBank]
Sequencing primer gene specific primer, 16vf: 5'-GATTATCGAAGATCTCCTTACCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117753-0022-292-G06C-16vf
CGAGATACCCCTCCCATCTCCGTCGCAAACTCGGCCTCTGTTTCACCAATTTGGAAATTT
AAAAACAGAGTAAGTTTCAACTCCCAGCGAGTTAAGAGAAAGTCAATTCCAAGAAAACCC
CTTTCAGATATTTTTTTCTTTCAAATTCGATCACAAACTAATGAAGAGAATCAAATTAAA
AAAATGGAGGCACCTTTCGTCGGAGGGTGAGGAGACAACGGCCGTGAGCGTCCATGAGGA
CAACCTCGTCGGTGTCGCGGGTGTTGGGACCACCGTAAGAGTCAACCCGAAACACCAAAC
TTCCCTTGCAGTCGTAAACTGTGAAACCATCGCCGGCGAAAAACAGAGATGTTTTCCGAA
CAGTCAGGCTCCTTTCTTCTCCGTGTATAAATTCATCTCCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 12-K117752-0022-292-G06C-8409
GenBank Accession LN491772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117752-0022-292-G06C-8409
GAGATGAATTTATACACGGAGAAGAAAGGAGCCTGACTGTTCGGAAAACATCTCTGTTTT
TCGCCGGCGATGGTTTCACAGTTTACGACTGCAAGGGAAGTTTGGTGTTTCGGGTTGACT
CTTACGGTGGTCCCAACACCCGCGACACCGACGAGGTTGTCCTCATGGACGCTCACGGCC
GTTGTCTCCTCACCCTCCGACGAAAGGTGCCTCCATTTTTTTAATTTGATTCTCTTCATT
AGTTTGTGATCGAATTTGAAAGAAAAAAATATCTGAAAGGGGTTTTCTTGGAATTGACTT
TCTCTTAACTCGCTGGGAGTTGAAACTTACTCTGTTTTTAAATTTCCAAATTGGTGAAAC
AGAGGCCGAGTTTGCGACGGAGATGGGAGGGGTATCTCGGGGAGAGATCGGACGGCCAGA
AACCGATCTTCGGGGTAAGGAG

Last Updated on 06.12.2018 16:11