Sequences from confirmation amplicon


Confirmation sequences for line 293A04 and hit At5g44280 are shown.
Line ID 293A04
Locus At5g44280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17838249
Primers used for PCR AC23 - 8474, show primer details
   
Sequence Name 15-K027788-022-293-A04C-8409
GenBank Accession LN492824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K027788-022-293-A04C-8409
AAATGCAGACACAATTAAACAAGAAAGATCCATCCAGCTTCTTTATTCTACAGTAAAGAA
TCTATCTCAAGACAAAAATAAAGCTGACATTCTAAAGGTCAAGTTAAATAGAAGATGAAT
CATTCTTAGGACACAACTTCCAGTAATGGCCGCTATTATTCAAGGTAAAGACTTTACATT
GTTTAGCCACAAGAGCGTAGAGGTTTTGAGCAGTAATACGAGAGGAATTACAACATGCAA
GTGCTAAGAAGATGCATCAACAGATTACTAAATCAAGCAGAGCAATTTTAACATTTAATT
GACTAGAACAACAGAAGTAAAGAAATATTGGAGTAGAGAACCCATCACAAGCTAAACACA
TCTGGAGAAGAGAAAGACAGAGAAATGAGAAAAATCATTACTCTGATTGGTCTCCAGATA
TAGAAGATGGACTTCTTTCTTTAGAATCTGAAACAGAAACAAAGAGAGAGAACCAAATCG
ATCACATGAACAAA
   
Sequence Name 16-K027788-022-293-A04C-AC23
GenBank Accession LN493292 [GenBank]
Sequencing primer gene specific primer, AC23: 5'-AAACGTGAGAGTGTTTTGTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K027788-022-293-A04C-AC23
TTTCTGTTTCAGATTCTAAAGAAAGAAGTCCATCTTCTATATCTGGAGACCAATCAGAGT
AATGATTTTTCTCATTTCTCTGTCTTTCTCTTCTCCAGATGTGTTTAGCTTGTGATGGGT
TCTCTACTCCAATATTTCTTTACTTCTGTTGTTCTAGTCAATTAAATGTTAAAATTGCTC
TGCTTGATTTAGTAATCTGTTGATGCATCTTCTTAGCACTTGCATGTTGTAATTCCTCTC
GTATTACTGCTCAAAACCTCTACGCTCTTGTGGCTAAACAATGTAAAGTCTTTACCTTGA
ATAATAGCGGCCATTACTGGAAGTTGTGTCCTAAGAATGATTCATCTTCTATTTAACTTG
ACCTTTAGAATGTCAGCTTTATTTTTGTCTTGAGATAGATTCTTTACTGTAGAATAAAGA
AGCTGGATGGATCTTTCTTGTTTAATTGTGTCTGCATTTTTTTCAGACGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11