Sequences from confirmation amplicon


Confirmation sequences for line 293A10 and hit At5g05990 are shown.
Line ID 293A10
Locus At5g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1807936
Primers used for PCR CQ44 - 8474, show primer details
   
Sequence Name 07-K106540-0022-293-A10C-8409
GenBank Accession LN489284 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106540-0022-293-A10C-8409
GCCTTTACAATTGAATATATACTGTTCTATTGATTTTCTTTTTCTTCAATTTGAGCTTCA
ACACAAAAAAAATGGCGACTCTTGTACGTCGCGCAGCTTCTCGGTTTGTGGGAAGTTATT
GGAAAAACCGTTCTTTCGTTTACTCATTGTTGGACCAGTCCGCTTCTGGCCGGAGGCGAC
ATCTGACGCCGTCGGTTGACCGGAATGTTCCATTTACCACATCGACCAAAAAGAGAGCTT
CTCCAACTGATCCGCTTCTTCGAATCATTGAAGAGGAGATTGGATACGCCGAGAAAGCTG
ATGATTATCATCGAGTAAATCCTTACCATTTTCATGTTTTAGTTTCTGCAAATTTTGTTT
ATTAAGATTTATGTGTTTCGTGAAGCTCTGTTTCAATGGCAATGGTGTGTAATCTCTCTG
TTTCTTCTTTTGTTTGAATAAAGGTTGAAGAAACTCCAAGTGGCTTCCCTTTCGAAATGG
AAGACAAACCAGGAGGGAAGATTGTGACATTGACTAGAGAATATGAAGGAGAGACTGTTA
AAGTTGAAGTACACATGACTAATCTTGTGACTGGTGACAAAGAAGATGATGAAGATGATG
AGGAAGAAGCAGAGAATGAAGA
   
Sequence Name 23-K106541-0022-293-A10C-CQ44
GenBank Accession LN496568 [GenBank]
Sequencing primer gene specific primer, CQ44: 5'-TTCTTCATTCTCTGCTTCTTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106541-0022-293-A10C-CQ44
GATTAGTCATGTGTACTTCAACTTTAACAGTCTCTCCTTCATATTCTCTAGTCAATGTCA
CAATCTTCCCTCCTGGTTTGTCTTCCATTTCGAAAGGGAAGCCACTTGGAGTTTCTTCAA
CCTTTATTCAAACAAAAGAAGAAACAGAGAGATTACACACCATTGCCATTGAAACAGAGC
TTCACGAAACACATAAATCTTAATAAACAAAATTTGCAGAAACTAAAACATGAAAATGGT
AAGGATTTACTCGATGATAATCATCAGCTTTCTCGGCGTATCCAATCTCCTCTTCAATGA
TTCGAAGAAGCGGATCAGTTGGAGAAGCTCTCTTTTTGGTCGATGTGGTAAATGGAACAT
TCCGGTCAACCGACGGCGTCAGATGTCGCCTCCGGCCAGAAGCGGACTGGTCCAACAATG
AGTAAACGAAAGAACGGTTTTTCCAATAACTTCCCACAAACCGAGAAGCTGCGCGACGTA
CAAGAGTCGCCATTTTTTTTGTGTTGAAGCTCAAATTGGAAGAAAAAGAAAATCAATATG
AACAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
Primers used for PCR CQ45 - 8474, show primer details
   
Sequence Name 08-K106540-0022-293-A10C-OriL
GenBank Accession LN489743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106540-0022-293-A10C-OriL
TCGGACTGAGCCATTATTTCCCTTTAGGGTTAATATAGCAAATTACAAATTTAGCGGGAC
TTAAATTAAAAATCACAGAAAATCAGGACCGGCTAAAAATACGGGCCAATATGGGCCTAG
TGGCCCATTACGACATTAAGAGACCGTTTCCATAATCCGAAACCCTAGTTTGCTTCTGTC
TTTAGTTGTTGCCTTTTAAACTAAACTAACGAACTTAATAAGCGGCATATCTAAATCTGA
GAAAAATGGCGATTGATACGATACAAAAAACAAAAAAGCTTCGGTGGGGAGAAATTGACG
AAGAAGAAGGCGACTATGATTTCCTTCTTCCGCCTAAGCAGATGATTAGTCCGGACCAGA
ACGGTGTAAAGAAGGTGATTGAGTACAAATTCAACGAAGAGGACAAAAAGGTCAAAATCA
CTACCACGACCCGTGTTCAGAAGCGAGCTCTCACCAAACAAGCCGTGGAGCGACGGAGCT
GGAATAAGTTCGGAGACGCAGCTCATGAAGAATCCAGTAGTTACCTCACAATGCGTTCAA
   
Sequence Name 24-K106541-0022-293-A10C-CQ45
GenBank Accession LN497005 [GenBank]
Sequencing primer gene specific primer, CQ45: 5'-GTTGAACGCATTGTGAGGTAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106541-0022-293-A10C-CQ45
CAACTTTTCAGCTCCGTCGCTCCCGGCTTGTTTGGTGAGAGCTCGCTTCTGAACACGGGT
CGTGGTAGTGATTTTGACCTTTTTGTCCTCTTCGTTGAATTTGTACTCAATCACCTTCTT
TACACCGTTCTGGTCCGGACTAATCATCTGCTTAGGCGGAAGAAGGAAATCATAGTCGCC
TTCTTCTTCGTCAATTTCTCCCCACCGAAGCTTTTTTGTTTTTTGTATCGTATCAATCGC
CATTTTTCTCAGATTTAGATATGCCGCTTATTAAGTTCGTTAGTTTAGTTTAAAAGGCAA
CAACTAAAGACAGAAGCAAACTAGGGTTTCGGATTATGGAAACGGTCTCTTAATGTCGTA
ATGGGCCACTAGGCCCATATTGGCCCGTATTTTTAGCCGGTCCTGATTTTCTGTGATTTT
TAATTTAAGTCCCGCTAAATTTGTAATTTGCTATATTAAACCCTAAAGGGAAATAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACAGCGTTATT

Last Updated on 06.12.2018 16:11