Genotyping and primer details


Genotyping and primer details for 293A10 - At5g05990
Line ID 293A10
Locus At5g05990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1807936 of line 293A10 Chr5:1807936
   
Insertion allele amplicon 8474 - CQ44
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ44: 5'-TTCTTCATTCTCTGCTTCTTCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1807344
   
Insertion allele amplicon 8474 - CQ45
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ45: 5'-GTTGAACGCATTGTGAGGTAACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1808464
   
wt amplicon (over InsPos) CQ45 - CQ44
wt amplicon start Chr5:1807344
wt amplicon end Chr5:1808464
wt amplicon size 1121 bp
Locus specific primer CQ44: 5'-TTCTTCATTCTCTGCTTCTTCCTC-3'
2nd locus specific primer CQ45: 5'-GTTGAACGCATTGTGAGGTAACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11