Sequences from confirmation amplicon


Confirmation sequences for line 293F03 and hit At2g19210 are shown.
Line ID 293F03
Locus At2g19210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8337895
Primers used for PCR EQ15 - 8474, show primer details
   
Sequence Name 04-K102864-0022-293-F03C-8409
GenBank Accession LN487870 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102864-0022-293-F03C-8409
GCCTTTACTGGAGGAGCTCTTCTTCCTCCATACTTGGACTTGATTCTCATAATTGCATCA
ACTAGAATTTATACCATAAATGGATAAGTATTTGAATAAATTACGAAAAGGGTTAAGTAT
TGTATTAGAAATGAAAGTGTACCATCTTGTTGATCAGTAGGTGACTGTAGAAACTCGTTG
ACACGATATGTTTCAATGGCGTTAATGATGGGTGGAAGGGTAGATCTAGGAGTTTGTTGA
AGACGAAACTCGAGTTTTGGTCCGCTCACGGGGTTCTGAACAAAAAGCGTGTCTGTATAC
AAGTATCGCGGGCTAAAGGAGCTCATGTTGATTTCCTTTTCGTTTAATAACACCTTAAAT
TCTCTTGTCTCATTACTTGGCAGCTCCACAACTTCTGCAAAGTGCATATACACAAAATAC
TTCCAAGTAGGATCCTTTGGTTCCCAAGAAAAGATTATGTCTTGGCTTAAATCCTCAGGT
GAAGTTGCAGTGTTCATAACAAAGCGAGCGGGTTGGAATCCCTCGTTGTTATTCGAATCT
TNGGTGAGCG
   
Sequence Name 04-K102869-0022-293-F03C-EQ15
GenBank Accession LN487871 [GenBank]
Sequencing primer gene specific primer, EQ15: 5'-CGCTCACAATAGATTCGAATAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102869-0022-293-F03C-EQ15
CGCTGCTTGTATGACACTGCAACTTCACCTGAGGATTTAAGCCAAGACATAATCCTTTAA
AGGGAACCAAAGGATCCTACTTGGAAGTATTTTGTGTATATGCACTTTGCAGAAGTTGTG
GAGCTGCCAAGTAATGAGACAAGAGAATTTAAGGTGTTATTAAACGAAAAGGAAATCAAC
ATGAGCTCCTTTAGCCCGCGATACTTGTATACAGACACGCTTTTTGTTCAGAACCCCGTG
AGCGGACCAAAACTCGAGTTTCGTCTTCAACAAACTCCTAGATCTACCCTTCCACCCATC
ATTAACGCCATTGAAACATATCGTGTCAACGAGTTTCTACAGTCACCTACTGATCAACAA
GATGGTACACTTTCATTTCTAATACAATACTTAACCCTTTTCGTAATTTATTCAAATACT
TATCCATTTATGGTATAAATTCTAGTTGATGCAATTATGAGAATCAAGTCCAAGTATGGA
GTGAAGAAGAGCTCCTCCAGTAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTCTTATNAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTACGACATGTCTACATTCACGTCCAAATGGG
Primers used for PCR KH50 - 3144, show primer details
   
Sequence Name 06-K106971-0022-293-F03C-3144
GenBank Accession LN488834 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106971-0022-293-F03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAG
TAAAATACCCTTGGAAGGATATTAACTGCAGCTATGTCGATAATGAGTCTCCCAGAATTA
TTTCTGTGTAAGCTCCTCTTCAAATGCTTGTATATGTCTTTGATCTGTATGTCTTTTTCT
CACAAGAGTTTGTTGATCTTCAGAAATTTATCCTCGAGTGGCTTGACTGGAGAGATTGAT
GCAGCGTTCTCAAACCTTACATTGTTACATATATTGTTCGTATTTTCGATTTGATATGGA
ATTCTTTTGTATACAAATTTGTGGAATTCTTACTGGATGCAA
   
Sequence Name 22-K106972-0022-293-F03C-KH50
GenBank Accession LN496134 [GenBank]
Sequencing primer gene specific primer, KH50: 5'-GTTGCATCAGTTAAGAATTCCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106972-0022-293-F03C-KH50
CAATCGAAATACGAACAATATATGTAACAATGTAAGGTTTGAGAACGCTGCATCAATCTC
TCCAGTCAAGCCACTCGAGGATAAATTTCTGAAGATCAACAAACTCTTGTGAGAAAAAGA
CATACAGATCAAAGACATATACAAGCATTTGAAGAGGAGCTTACACAGAAATAATTCTGG
GAGACTCATTATCGACATAGCTGCAGTTAATATCCTTCCAAGGGTATTTTACTCAAACAC
TGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATATCAATCAAATCCAC

Last Updated on 06.12.2018 16:11