Sequences from confirmation amplicon


Confirmation sequences for line 294B04 and hit At3g15355 are shown.
Line ID 294B04
Locus At3g15355 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5186415
Primers used for PCR 11vi - 8474, show primer details
   
Sequence Name 25-K117806-0022-294-B04C-11vi
GenBank Accession LN497643 [GenBank]
Sequencing primer gene specific primer, 11vi: 5'-TTGTCTCGATCCAAGGTCTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117806-0022-294-B04C-11vi
CCGGCTCGAGAGGTCAGCAGGTTCTGCACATGGTGAGAGTACCTCTAAAGCTTACAGCGA
AAACACATTCATACTGTCTTTAAAGACTATGGTTTACACCATGAGGAGACCACCCAAGGT
AAAAGCAAAAACCTCATCCCATTACACTTGTCTCTCTCTACTAATAAAAAAGCGTAAACC
TTTTCACTAAAACTTGAGATTGATTGTTTCTGGTGGTTTTTCGAATGCTACAGTATTTTG
AAGACTTTGCGTATGGACATTTCTTCAGCTGTGCTCACGACGTGTTGAAGGCGTGTAATG
CTTACAGAAACGGAGCTACACCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACAGTTATTAT
   
Sequence Name 25-K117784-0022-294-B04C-8409
GenBank Accession LN497642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117784-0022-294-B04C-8409
TCGTTCTGTAGCATTACACGCCTTCAACACGTCGTGAGCACAGCTGAAGAAATGTCCATA
CGCAAAGTCTTCAAAATACTGTAGCATTCGAAAAACCACCAGAAACAATCAATCTCAAGT
TTTAGTGAAAAGGTTTACGCTTTTTTATTAGTAGAGAGAGACAAGTGTAATGGGATGAGG
TTTTTGCTTTTACCTTGGGTGGTCTCCTCATGGTGTAAACCATAGTCTTTAAAGACAGTA
TGAATGTGTTTTCGCTGTAAGCTTTAGAGGTACTCTCACCATGTGCAGAACCTGCTGACC
TCTCGTAGCCGGGCTCGTTGAAGTAAGGTTTTTGATTTAAGATTAGACCTTGGATCGAGA
CAA

Last Updated on 06.12.2018 16:11