Sequences from confirmation amplicon


Confirmation sequences for line 294D06 and hit At3g21600 are shown.
Line ID 294D06
Locus At3g21600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7607718
Primers used for PCR 50ql - 8474, show primer details
   
Sequence Name 80-K115459-0022-294-D06C-50ql
GenBank Accession LN513983 [GenBank]
Sequencing primer gene specific primer, 50ql: 5'-ATGGGATGTTTCGGACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K115459-0022-294-D06C-50ql
GACAGATAACCGACAAACCCCTCTCATAACCTCAAACCATGAGAGCTGAAGAAGTTCTGT
TGCAGATTCCTAGATGTAGAGTCCATCTCATAGGCGAATCCGAGGCGGTGGAGCTTGCCT
CTGGTGATTTCAAGCTCGTTAAGGTCTCAGACAACGGTGTAACTCTAGCTATGATCGTGA
GAATCGGACATGACCTTCAGTGGCCAGTGATTAGAGATGAGCCAGTGGTAAAACTTGACG
CCCGTGATTACCTCTTCACTCTCCCGGTTAAAGACGGTGATCCACTTAGCTACGGGGTCA
CTTTCTCTGGCGATGACAGAGACGTAGCCCTCGTGAACAGTCTGAAGTTGCTTGACCAAT
TCTTGAGTGAGAATTCTTGTTTCTCGTCTACGGCTTCGAGTAAGGTTAACAATGGAATCG
ACTGGCAAGAGTTTGCGCCGAGGATTGAAGATTACAACAACGTTGTTGCTAAGGCTATTG
CTGGAGGAACAGGACATATCATTAGAGGAATCTTTAGTCTCAGTAATGCTTACTCTAACC
AGGTTTAAATCACACCCTCATGATATATTCGAAGCTCTCTTGATATATATATTTCCCTCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 31-K115458-0022-294-D06C-8409
GenBank Accession LN500291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115458-0022-294-D06C-8409
GCCATTTCATTGAATATATCCTGAGGGAAATATATATATCAAGAGAGCTTCGAATATATC
ATGAGGGTGTGATTTAAACCTGGTTAGAGTAAGCATTACTGAGACTAAAGATTCCTCTAA
TGATATGTCCTGTTCCTCCAGCAATAGCCTTAGCAACAACGTTGTTGTAATCTTCAATCC
TCGGCGCAAACTCTTGCCAGTCGATTCCATTGTTAACCTTACTCGAAGCCGTAGACGAGA
AACAAGAATTCTCACTCAAGAATTGGTCAAGCAACTTCAGACTGTTCACGAGGGCTACGT
CTCTGTCATCGCCAGAGAAAGTGACCCCGTAGCTAAGTGGATCACCGTCTTTAACCGGGA
GAGTGAAGAGGTAATCACGGGCGTCAAGTTTTACCACTGGCTCATCTCTAATCACTGGCC
ACTGAAGGTCATGTCCGATTCTCACGATCATAGCTAGAGTTACACCGTTGTCTGAGACCT
TAACGAGCTTGAAATCACCAGAGGCAAGCTCCACCGCCTCGGATTCGCCTATGAGATGGA
CTCTACATCTAGGAATCTGCAACAGAACTTCTTCAGCTCTCATGGTTTGAGGTTGATGAG
AGGGGTTTTGTCGTGTTATCTTGTCATGTTCATTGTTTCTTGTTCTTGAAGCCTTTGATG
GTCCGAACCATCCC

Last Updated on 06.12.2018 16:11