Sequences from confirmation amplicon


Confirmation sequences for line 295A05 and hit At5g50170 are shown.
Line ID 295A05
Locus At5g50170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20421787
Primers used for PCR 14dl - 8474, show primer details
   
Sequence Name 28-K109778-0022-295-A05C-14dl
GenBank Accession LN498889 [GenBank]
Sequencing primer gene specific primer, 14dl: 5'-CATTAAGAACCTTCTCACCAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K109778-0022-295-A05C-14dl
ACTCAGAAGAATTTTCCCTACAATACAAAGGTAAAGTTCATATCCAAAACCTCCCTATAC
ACACACACAGATACAGAGCCCAATTCCTAATTCACTAATGATAACACACACACACACACA
CACACAATTGATTCACTAACTAGAGACTTAAAATTCTCACCTAACACCAGAAAACACCAT
TTCAAATGAATCTACAAGTTACTTAACCAATGCTCAAAGCATGAATCACCAAAGAAGACA
CAAACATGCCATTTCTTCTCATGCTTAAACACCAACACCAGAAAACAACACCTCCATATA
CAGAGACTTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTATATGTCCGCAGCGTTAAT
   
Sequence Name 12-K109777-0022-295-A05C-8409
GenBank Accession LN491623 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109777-0022-295-A05C-8409
TTGAATTTAGTCTCTGTATATGGAGGTGTTGTTTTCTGGCCTTGAAGTTTAAGCATGAGA
AGAAATGGCATGTTTGTGTCTTCTTTGGTGATTCATGCTTTGAGCATTGGTTAAGTAACT
TGTAGATTCATTTGAAATGGTGTTTTCTGGTGTTAGGTGAGAATTTTAAGTCTCTAGTTA
GTGAATCAATTGTGTGTGTGTGTGTGTGTGTGTTATCATTAGTGAATTAAGAATTGGGCT
CTGTATCTGTGTGTGT

Last Updated on 06.12.2018 16:11