Sequences from confirmation amplicon


Confirmation sequences for line 295F02 and hit At3g04450 are shown.
Line ID 295F02
Locus At3g04450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1184441
Primers used for PCR PF52 - 8474, show primer details
   
Sequence Name 39-K103737-0022-295-F02C-PF52
GenBank Accession LN503327 [GenBank]
Sequencing primer gene specific primer, PF52: 5'-AAAGGCAAAACAAAAAGTGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103737-0022-295-F02C-PF52
GAAGGGAATAATATAGGATATTAACTAGGCGAATGGAATTTTTTCCTCATACAGAGTCAT
TTTCTGTATATATAACTTAGTGATTGTGAGTTCTCATATTGCTTCTATGTAACACTCCTT
GTAAAGCTAAATAGCCCTTTTGTGCTAAGTCTCTGATATCTATTGTTATCTTCTCAATAC
TGCACTTGTTCAATGACACTTGCTAGCGATTTTGGTTTTCCATCCGCTATATCTTCATCT
TTTACAATTCTAGAAGAGAGATACCACAATAACTTTCCTAACACTTTGTGTGTTTCATCA
GGACAGGAATCGATGAATAACAACCCCGTCCCTGAGATATTCAATGGAGTAATGGCTATT
TAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGA
   
Sequence Name 55-K103738-0022-295-F02C-8409
GenBank Accession LN508787 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103738-0022-295-F02C-8409
ATCCGGACTGAGCCTTTACAATTGAATATATCCTAAATAGCCATTACTCCATTGAATATC
TCAGGGACGGGGTTGTTATTCATCGATTCCTGTCCTGATGAAACACACAAAGTGTTAGGA
AAGTTATTGTGGTATCTCTCTTCTAGAATTGTAAAAGATGAAGATATAGCGGATGGAAAA
CCAAAATCGCTAGCAAGTGTCATTGAACAAGTGCAGTATTGAGAAGATAACAATAGATAT
CAGAGACTTAGCACAAAAGGGCTATTTAGCTTTACAAGGAGTGTTACATAGAAGCAATAT
GAGAACTCACAATCACTAAGTTATATATACAGAAAATGACTCTGTATGAGTGAAAAAATT
CCATTCGCCTAGTTAATATCCTATATGTATTTCCCTTTCAAAACTTTCCAAAGGAATATT
CTTCACTTTTTGTTTTGCCT

Last Updated on 06.12.2018 16:11