Sequences from confirmation amplicon


Confirmation sequences for line 295G05 and hit At3g03150 are shown.
Line ID 295G05
Locus At3g03150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:728532
Primers used for PCR 14oz - 8474, show primer details
   
Sequence Name 58-K115121-0022-295-G05C-14oz
GenBank Accession LN509785 [GenBank]
Sequencing primer gene specific primer, 14oz: 5'-TCTGGCTTTATAACTTCGTCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K115121-0022-295-G05C-14oz
TCTTGTCATACGCCGAGGAATGTCCACTCCGACTGTTAAAAAGGAAGGAAATAAAAAGAA
TATTTCAGACGAATTTTATATCTAACCGACCTCAATTTATTAATATGATAATTTCGGTAT
CAATTTTATTAATCATATCAAGGAAATAAAAACAAATTTCCTGTTGGGTGGGAGATTGTT
GCTAGGTAGGTGAGACATTGCCACCACAAACCCACAACAACCCCAAAAAGAATGAATTAT
TACGAAGATGGTCTTTATATAGATTTTTTCTTTATCAGGTCTTAGTATATTATTAAAGAT
GGAAAGTTTGGTTTTTCATAAAAAGTGACCCCGTAAATGCCTTTTCTTTAAAAATTAAAA
ATAAAATCAAGTAACACCAAATATGTCATAGTCAGCTCCATCAACATTTCTATATCTTCA
AAGCTGTCGGTCCAAATTCTTATCTTAAATTAACGCTATGAGAAAATCAGCCACCTCATG
TCTACTCATATCANAAATGAAAAATAATGTGTTACACTGTTACGAAAAGATGGACTACAC
AGCANGTAACTAAATGTGGCTTCATATTGAAGATGATAAACAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K115120-0022-295-G05C-8409
GenBank Accession LN490783 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115120-0022-295-G05C-8409
TTGAATATATCCTGTTTATCATCTTCAATATGAAGCCACATTTAGTTACATGCTGTGTAG
TCCATCTTTTCGTAACAGTGTAACACATTATTTTTCATTTATGATATGAGTAGACATGAG
GTGGCTGATTTTCTCATAGCGTTAATTTAAGATAAGAATTTGGACCGACAGCTTTGAAGA
TATAGAAATGTTGATGGAGCTGACTATGACATATTTGGTGTTACTTGATTTTATTTTTAA
TTTTTAAAGAAAAGGCATTTACGGGGTCACTTTTTATGAAAAACCAAACTTTCCATCTTT
AATAATATACTAAGACCTGATAAAGAAAAAATCTATATAAAGACCATCTTCGTAATAATT
CATTCTTTTTGGGGTTGTTGTGGGTTTGTGGTGGCAATGTCTCACCTACCTAGCAACAAT
CTCCCACCCAACAGGAAATTTGTTTTTATTTCCTTGATATGATTAATAAAATTGATACCG
AAATTATCATATTAATAAATTGAGGTCGGTTAGATATAAAATTCGTCTGAAATATTCTTT
TTATTTCCTTCCTTTTTAACAGTCGGAGTGGACATTCCTCGGCGTATGACAAGAACGTGG
AGGATGAATTGCATGCAAGTGCAGTACCTGACGAAGTTTAAAAGCCAG

Last Updated on 06.12.2018 16:11