Sequences from confirmation amplicon


Confirmation sequences for line 296F04 and hit At5g07250 are shown.
Line ID 296F04
Locus At5g07250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2275979
Primers used for PCR FV83 - 8474, show primer details
   
Sequence Name 15-K100894-0022-296-F04C-8409
GenBank Accession LN492894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K100894-0022-296-F04C-8409
GCCTTTACATTGAATATATCCTGACAAAAAACAATAAACAAATTACAGACAGAGAAGAGA
GAGATCACTATGGCTGTCGGAGATGATGATCTGGAGAACAGAATGTCGGCGAAAGATCGA
GGAATCGGAAGCAGAGGCGGTGATCGGAATCGAATCGGACCACCGCCTCTGCCGGTAGCC
TTGTCGTCGTCTACTGAATTTGGCGATAACGCGTTGTCGTCGCGGTGGACGTCGTGGCTT
GTTCCGATGTTCGTTGTGGCTAACGTTGCGGTTTTCGTCGTGGCGATGTTCGTTAATAAC
TGTCCGAATCATTTCGAGTCTCACCGTCTCAGAGGTCACTGTGTCGCCNAGTTTCTCGGT
AGACTCTCCTTTGAGCCTCTCCGAACTAATCCTCTCTNTGGTCCATCTNCTCACACGTAC
GTTTTCTAAATTTTGTATCGATGTTGATTTAGGGTTCTAAATACTAGAGAATCACGTTTT
GGTTCTGTTTCTCAAGTTTGTCGGTTACTAAATCGAAATTTACTGGATCTTGA
   
Sequence Name 15-K100902-0022-296-F04C-FV83
GenBank Accession LN492895 [GenBank]
Sequencing primer gene specific primer, FV83: 5'-TCATTTCAACATCTCAAGATCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K100902-0022-296-F04C-FV83
CGACAACTTGAGAACAGAACCAAAACGTGATTCTCTAGTATTTAGAACCCTAAATCAACA
TCGATACAAAATTTAGAAAACGTACGTGTGAGAAGATGGACCAAAGAGAGGATTAGTTCG
GAGAGGCTCAAAGGAGAGTCTACCGAGAAACTTGGCGACACAGTGACCTCTGAGACGGTG
AGACTCGAAATGATTCGGACAGTTATTAACGAACATCGCCACGACGAANACCGCAACGTT
AGCCACAACGAACATCGGAACAAGCCACGACGTCCACCGCGACGACNACGCGTTATCGCC
AAATTCAGTAGACGACGACNAGGCTACCGGCAGAGGCGGTGGTCCGATTCGATTCCGATC
ACCGCCTCTGCTTCCGATTCCTCGATCTTTCGCCGACATTCTGTTCTCCAGATCATCATC
TCCGACAGCCATAGTGATCTCTCTCTTCTCTGTCTGTAAATTGTTTATTGTTTTTTTTGT
CAGGATATATTCAATTGTAAATGGCTTCATGTCCNGGAAATCTACCTGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATG

Last Updated on 06.12.2018 16:11