Sequences from confirmation amplicon


Confirmation sequences for line 296H02 and hit At4g02330 are shown.
Line ID 296H02
Locus At4g02330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1032874
Primers used for PCR 14of - 8474, show primer details
   
Sequence Name 05-K114782-0022-296-H02C-14of
GenBank Accession LN488460 [GenBank]
Sequencing primer gene specific primer, 14of: 5'-CTTCCTTCAAACTCTTCATCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114782-0022-296-H02C-14of
GATCAAATTCTGCAATCAGTGTTCCCACAACATCTCAAGGCGACGTTCGCGAATACGGCC
GCTTCTCCCTCCGCAAATCGCTAACGCAATCGCGGAAATTCACACGCACCATTGACAGAT
ACCTCAAACGCAACAATGCACTATTATCTCAATCCGCCGTTGGAGCTCTCCAAGACTGCC
GTTACCTAGCAAGCCTCACAACAGACTATCTCATAACATCGTTCGAGACGGTCAATATCA
CTACATCATCCAAAACGTTGTCGTTTTCCAAAGCCCATTACCTACATGGCTTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACACGAT
TATA
   
Sequence Name 05-K114769-0022-296-H02C-8409
GenBank Accession LN488459 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114769-0022-296-H02C-8409
TTCATTGAGCCATGTAGGTAATGGGCTTTGGAAAACGACACGTTTTGGATGATGTAGTGA
TATTGACCGTCTCGAACGATGTTATGAGATAGTCTGTTGTGAGGCTTGCTAGGTAACGGC
AGTCTTGGAGAGCTCCAACGGCGGATTGAGATAATAGTGCATTGTTGCGTTTGAGGTATC
TGTCAATGGTGCGTGTGAATTTCCGCGATTGCGTTAGCGATTTGCGGAGGGAGAAGCGGC
CGTATTCGCGAACGTCGCCTTGAGATGTTTGTGGGAACACTGATTTGCAGAATTTTGGAT
CGGGTGTTGTTTTGCATATGGTTGATGAAGAGTTTGAAGGAAG

Last Updated on 06.12.2018 16:11