Sequences from confirmation amplicon


Confirmation sequences for line 297B10 and hit At5g67060 are shown.
Line ID 297B10
Locus At5g67060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26766833
Primers used for PCR PH69 - 8474, show primer details
   
Sequence Name 16-K017544-022-297-B10C-PH69
GenBank Accession LN493216 [GenBank]
Sequencing primer gene specific primer, PH69: 5'-ACCACAACAACACTTACCCTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017544-022-297-B10C-PH69
CTCCACTCATTACCAGTCCGATCACTCAATGACCAACGAACCAGGTTTCCGCTACGGTTC
CGGTTTACTCACTAACCCTTCTTCTATCTCTCCCAACACAGCTTACTCTTCCGTTTTTCT
TGACAAAAGAAACAACAGTAACAACAACAATAATGGCACGAACATGGCAGCTATGCGAGA
GATGATCTTCCGTATCGCCGTGATGCAACCGATCCATATCGATCCCGAGGCGGTTAAGCC
ACCGAAGAGGAGGAACGTCAGGATCTCTAAAGATCCTCAAAGCGTGGCGGCTAGGCATAG
AAGGGAGAGAATAAGCGAGAGGATTCGGATTTTGCAACGGCTTGTTCCTGGTGGGACGAA
GATGGATACAGCTTCGATGCTCGATGAAGCAATTCATTATGTGAAGTTTTTAAAGAAACA
GGTGCAGTCTCTGGAGGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 20-K017544-022-297-B10C-8409
GenBank Accession LN495005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K017544-022-297-B10C-8409
CATGTAGATTTCTCCTCCAGAGACTGCACCTGTTTCTTTAAAAACTTCACATAATGAATT
GCTTCATCGAGCATCGAAGCTGTATCCATCTTCGTCCCACCAGGAACAAGCCGTTGCAAA
ATCCGAATCCTCTCGCTTATTCTCTCCCTTCTATGCCTAGCCGCCACGCTTTGAGGATCT
TTAGAGATCCTGACGTTCCTCCTCTTCGGTGGCTTAACCGCCTCGGGATCGATATGGATC
GGTTGCATCACGGCGATACGGAAGATCATCTCTCGCATAGCTGCCATGTTCGTGCCATTA
TTGTTGTTGTTACTGTTGTTTCTTTTGTCAAGAAAAACGGAAGAGTAAGCTGTGTTGGGA
GAGATAGAAGAAGGGTTAGTGAGTAAACCGGAACCGTAGCGGAAACCTGGTTCGTTGGTC
ATTGAGTGATCGGACTGGTAATGAGTGGAGTTAAAGAGAAAAGGGTAAGTG
Primers used for PCR 13bd - 3144, show primer details
   
Sequence Name 01-K107108-0022-297-B10C-3144
GenBank Accession LN486595 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K107108-0022-297-B10C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATCGGTGGAG
GTGGAATGACGGCGGCGAGTGGTGGTGGTGGCGGCGGGGGAGTGGTTATGAAAGGGTGTG
GAACAGTGGGGACTCATCAGATGGTGGGCAATGCACAGATTCTTAGATGATGATGATTTT
TAATTTTATTATTATTATATTAATGTTGGAGAAAAAGAGAAAAATGATTCTGGAGAGGGA
AGCCAAGTAATTTATGTGAGAGTCTTTAATTTAACTTTATTTTCTTGTTTAGATAATGTG
TAATGATGGTTTTTAAAGCCAAAGACTCTCCATGGTTGTTGGAGCGAGTTTGTAAATTAG
G
   
Sequence Name 17-K107113-0022-297-B10C-13bd
GenBank Accession LN493874 [GenBank]
Sequencing primer gene specific primer, 13bd: 5'-CCTAATTTACAAACTCGCTCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K107113-0022-297-B10C-13bd
CCATCATTACACATTATCTAAACAGAAAATAAAGTTAAATTAAAGACTCTCACATAAATT
ACTTGGCTTCCCTCTCCAGAATCATTTTTCTCTTTTTCTCCAACATTAATATAATAATAA
TAAAATTAAAAATCATCATCATCTAAGAATCTGTGCATTGCCCACCATCTGATGAGTCCC
CACTGTTCCACACCCTTTCATAACCACTCCCCCGCCGCCACCACCACCACTCGCCGCCGT
CATTCCACCTCCACCGATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAGT
CCACA

Last Updated on 06.12.2018 16:11