Sequences from confirmation amplicon


Confirmation sequences for line 297H09 and hit At3g07990 are shown.
Line ID 297H09
Locus At3g07990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2552679
Primers used for PCR BV07 - 8474, show primer details
   
Sequence Name 14-K105564-0022-297-H09C-BV07
GenBank Accession LN492505 [GenBank]
Sequencing primer gene specific primer, BV07: 5'-GGTAGAAACTGAAACCTGCATAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K105564-0022-297-H09C-BV07
TGTGGAACCTCTCAACCAGTTGACAAGAAATATGTACGAATCCTCAGCTACAGATTCATC
AAGACAAGACATTAAATAAAGCTCAAGATAGCCTGATTGAGGTAATAAACTTCAGACAAA
TATATTCAAAAAGCAGAACCTGTTCTCTGATCACCGGTTGTGTAAAGATCTGAAGTTGTG
TTTGAATATGAGAAACCAACTCCAGCTGGAGACTCCAAGAATAGCAAGTTTGCCACTGAG
AGAAAGAGACAAAGAACAAATCTGAATCGGAATCGTGAAGAGAAGAGCAAGATATGGTGA
GAGATTTAGTTAAAGATGGATATTACATTTATTCCAAGCATAAAGTTTGGAATGAAGAGT
CTTGCCATCAGAACCAACACGAAAAGGTCCAATTTCTTCAGCAGCACCATAAGCAACAGA
AGAACAACCAGGGCCACCATTGAGCCACAGAACCAAAGGTCTAGACTTTGGGTCACGGGC
CAACGGAGACTCGACCAACCAGTAGAACAAAGCTCTTCCACGTTCTTCATGCACAGTGAC
ATAGCCTGAGTACTGTCTAAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 62-K105565w-0022-297-H09C-OriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K105565w-0022-297-H09C-OriL
CATTTACATTGAATATATCCTGATTTAGACAGTACTCAGGCTATGTCACTGTGCATGAAG
AACGTGGAAGAGCTTTGTTCTACTGGTTGGTCGAGTCTCCGTTGGCCCGTGACCCAAAGT
CTAGACCTTTGGTTCTGTGGCTCAATGGTGGCCCTGGTTGTTCTTCTGTTGCTTATGGTG
CTGCTGAAGAAATTGGACCTTTTCGTGTTGGTTCTGATGGCAAGACTCTTCATTCCAAAC
TTTATGCTTGGAATAAATGTAATATCCATCTTTAACTAAATCTCTCACCATATCTTGCTC
TTCTCTTCACGATTCCGATTCAGATTTGTTCTTTGTCTCTTTCTCTCAGTGGCAAACTTG
CTATTCTTGGAGTCTCCAGCTGGAGTTGGTTTCTCATATTCAAACACAACTTCAGATCTT
TACACAACCGGTGATCAGAGAACAGGTTCTGCTTTTTGAATATATTTGTCTGAAGTTTAT
TACCTCAATCAGGCTATCTTGAGCTTTATTTAATGTCTTGTCTTGATGAATCTGTAGCTG
AGGATTCGTACATATTTCTTGTCAACTGGTTTGAGAGGTTTCCACAATACAAGCATAGGG
AGTTTTACATTGTTGGAGAAAGCTATGCA
Primers used for PCR BV06 - 8474, show primer details
   
Sequence Name 61-K105565w-0022-297-H09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K105565w-0022-297-H09C-8409
GCCATTTACATGGTAAGTTACTGATTCTGTCTCTCAATTGTTCTTCCCATAAGAAGAAGG
AGCAGCACAACATGAAGTAGAGATTGTGAGTAAGAGAATGATTAGAAGGAAAGAGTAATC
CATGATAATCAGATCTAAGAGAGTTAGATCTGATGGAACTCTGGATTTCCTCACGATTTG
GAGGTTTTACAGACACATTTACTATTTCTCGAAGTCAAACACTAACCTTACAGTTACTTA
ACAGAACAGCGCACTACTACTACTGTCTTGTCGTCAGACTCAAGTTTTTCTTTTTGGATT
GGTCAAACGAAAAATAACTTTTTTAACTTGACAGTCTTTTCATATTTTACCCAATTTCTA
GGATGGGCTTTTTTATCCGACATTATTTTGGTCTGACATGATGGGCAATTGGGCATGCCT
GATTTTTATCGAGGCTAGAACTAAATTGTCTTTTTTTTTTTTGGGGGGTTCCGGGAAAAA
AAAAAAAAATCCATTCCCCAAATTTGGATTTTTTTTTTTTTTTTTTTACCCCCGGGCCAG
GGGAAAGGGGAAAAACCCCAATTTTT

Last Updated on 06.12.2018 16:11