Sequences from confirmation amplicon


Confirmation sequences for line 298B01 and hit At3g19570 are shown.
Line ID 298B01
Locus At3g19570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6800129
Primers used for PCR 24cy - 8474, show primer details
   
Sequence Name 30-K109134-0022-298-B01C-24cy
GenBank Accession LN499755 [GenBank]
Sequencing primer gene specific primer, 24cy: 5'-ACCACTAACTGGAAGACGAAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K109134-0022-298-B01C-24cy
AAGAATTTGAGTGATTTCTGTCCAAAGAGACCATTCTTCTAGATAACACATCTACAATTC
CCAAATACAATTATTTCGTCAGTCTCAAAAAGGTTACAACATTTTAAAAAACATTTCTAT
GAATGTCATTTTTTTTTCTATGAATATGTCATAATCTCTCTTTTTTACAAGTGAATGTCA
GCAATTAAAAAAACTTTTGAATTTTGAAATAAATATGCTTTCATGTTTCTTAACGTGCTT
CCTTGTCTAAGATTCAGAATGCCATGAGATGATGATTTTAAGCAATCAAGATAATAACTT
CCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCG
   
Sequence Name 14-K109133-0022-298-B01C-8409
GenBank Accession LN492546 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K109133-0022-298-B01C-8409
CATTTACATTGGAAGTATTATCTTGATTGCTTAAAATCATCATCTCATGGCATTCTGAAT
CTTAGACAAGGAAGCACGTTAAGAAACATGAAAGCATATTTATTTCAAAATTCAAAAGTT
TTTTTAATTGCTGACATTCACTTGTAAAAAAGAGAGATTATGACATATTCATAGAAAAAA
AAATGACATTCATAGAAATGTTTTTTAAAATGTTGTAACCTTTTTGAGACTGACGAAATA
ATTGTATTTGGGAATTGTAGATGTGTTATCTAGAAGAATGGTCTCTTTTGGACAGAAATC
ACTCAAATTCTTTGTCAGGAGCAACTGAAGCCTTGAAAGCCAGCACACTTCGTCTTCCAG
TTAGTGG

Last Updated on 06.12.2018 16:11