Genotyping and primer details


Genotyping and primer details for 298B01 - At3g19570
Line ID 298B01
Locus At3g19570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6800129 of line 298B01 Chr3:6800129
   
Insertion allele amplicon 8474 - 24cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24cy: 5'-ACCACTAACTGGAAGACGAAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6800488
   
wt amplicon (over InsPos) 40dy - 24cy
wt amplicon start Chr3:6799533
wt amplicon end Chr3:6800488
wt amplicon size 956 bp
Locus specific primer 24cy: 5'-ACCACTAACTGGAAGACGAAGTGT-3'
2nd locus specific primer 40dy: 5'-AGTTTCTACGTTTTGGATTGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11