Sequences from confirmation amplicon


Confirmation sequences for line 298B11 and hit At3g62240 are shown.
Line ID 298B11
Locus At3g62240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23035377
Primers used for PCR 40qa - 8474, show primer details
   
Sequence Name 52-K115305-0022-298-B11C-40qa
GenBank Accession LN507910 [GenBank]
Sequencing primer gene specific primer, 40qa: 5'-AGAAAGAGGAGGAAAAGCAGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K115305-0022-298-B11C-40qa
GATCCGTGAAGAGGGTTCCATATCTGTAGTTGATAGAGATTCCATTGGCTGAATAAGGGG
ATCAGTATCATCCACGTGTATGTTGTTGTTTTCAGAGAATCCGGGTGGTGCACTTGAGGG
TGGAGGAGCAGGCTCTTGCCGAGAATACTCAGACTCAGATAAACGAAGAGCGGCATGAAC
TGCAAGATTATATTCTTCATCACCAGGCTCGCGACGAAATGATCGGGGTCTTCCTCGACG
ATTCTCTTGATCATTTCCACGACTGTATCGGAAACTTGTCGGTATCTGGAGTTGAATCCA
ATTGTTAAACACAGGCAATCAAACTGGACCATAAATTAACAATGACAACATGCAAGAGGT
GTTACATACCTGTAATGCAGCACTTCGCTGAGACCGAGACATTTTACCTCCATGCTCAAT
AGCGTTATGCCTCTGAAAAAAGAATAGAAATCCTCAACAAAAGTCTATGGAGTCAAACTA
AAAAAAAAGATACAGGAGAAATTCTTTAAAAGTGTTTACTTTCAACTCTGATTCATTCTG
GAAAACTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 17-K115304-0022-298-B11C-8409
GenBank Accession LN493980 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115304-0022-298-B11C-8409
AGTAAACACTTTTAAGAATTTCTCCTGTATCTTTTTTTTTAGTTTGACTCCATAGACTTT
TGTTGAGGATTTCTATTCTTTTTTCAGAGGCATAACGCTATTGAGCATGGAGGTAAAATG
TCTCGGTCTCAGCGAAGTGCTGCATTACAGGTATGTAACACCTCTTGCATGTTGTCATTG
TTAATTTATGGTCCAGTTTGATTGCCTGTGTTTAACAATTGGATTCAACTCCAGATACCG
ACAAGTTTCCGATACAGTCGTGGAAATGATCAAGAGAATCGTCGAGGAAGACCCCGATCA
TTTCGTCGCGAGCCTGGTGATGAAGAATATAATCTTGCAGTTCATGCCGCTCTTCGTTTA
TCTGAGTCTGAGTATTCTCGGCAAGAGCCTGCTCCTCCACCCTCAAGTGCACCACCCGGA
TTCTCTGAAAACAACAACATACACGTGGATGATACTGATCCCCTTATTCAGCCAATGGAA
TCTCTATCAACTACAGATATGGAACCCTCTTCACGGTATCTTCAAGCTGTAGGAAGCTTT
GGTGGTGGTGGTTCACGTCTTGGAGAATCTGCTTTTCCTCCCTCTTT

Last Updated on 06.12.2018 16:11