Sequences from confirmation amplicon


Confirmation sequences for line 298E10 and hit At2g05710 are shown.
Line ID 298E10
Locus At2g05710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2143668
Primers used for PCR ME15 - 8474, show primer details
   
Sequence Name 45-K027488-022-298-E10C-8409
GenBank Accession LN505405 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K027488-022-298-E10C-8409
CATTTACAATTGAATATATCCTGCAATTGAATGTTTAAACATATTGTTAGCCCGAAGATA
TGCTTCAATCATAGCCACCTGCCATGAAAGTATTGTATCAGACCAGATATCTGAATATAT
ATAGAAGGAAGATTAAAGTCAAACACTCACTGTCTCATCGCTTCTTCCAGTCAATTTGAG
ATATTGTAGAGTAACATGATCCACAGGGAAGAAGCCCATGGTTGCACCATACTCGGGAGA
CATATTGGCAATTGTCGCCCTGTCAGCTAGGGACAGCCCACTCATGCCATTACCTAAGAA
GAAAAAAGTAGACTTATAATGCTCCAACAAACACATGTCTATTATAAAAGAGCAAAACAC
TTAAGAGCTCACAAGGATATAATTACCATAAAACTCAACAAACTTTCCAACAACGCCATG
CTTCCTCAGCATTTGAGTCACTGTTAGAACCAAATCAGTAGCTGTTACACCATTACGCAT
TTTTCCGGCAAGTTTGAATCCAACAACACCAGGCAATACCATACTCATGGGCTGTCAAAC
CAAAAAAGCGTTATCAGTATAAGAAAAATTCACAAACC
   
Sequence Name 46-K027488-022-298-E10C-ME15
GenBank Accession LN505747 [GenBank]
Sequencing primer gene specific primer, ME15: 5'-CTCCAATACAGTGAGGTTTGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K027488-022-298-E10C-ME15
CTTTTTTGGTTTGACGCCCATGAGTATGGTATTGCCTGGTGTTGTTGGATTCCACTTGCC
GGAAAAATGCGTAATGGTGTAACAGCTACTGATTTGGTTCTAACAGTGACTCAAATGCTG
AGGAAGCATGGCGTTGTTGGAAAGTTTGTTGAGTTTTATGGTAATTATATCCTTGTGAGC
TCTTAAGTGTTTTGCTCTTTTATAATAGACATGTGTTTGTTGGAGCATTATAAGTCTACT
TTTTTCTTCTTAGGTAATGGCATGAGTGGGCTGTCCCTAGCTGACAGGGCGACAATTGCC
AATATGTCTCCCGAGTATGGTGCAACCATGGGCTTCTTCCCTGTGGATCATGTTACTCTA
CAATATCTCAAATTGACTGGAAGAAGCGATGAGACAGTGAGTGTTTGACTTTAATCTTCC
TTCTATATATATTCAGATATCTGGTCTGATACAATACTTTCATGGCAGGTGGCTATGATT
GAAGCATATCTTCGGGCTAACAATATGTTTAAACATTCAATTGCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11