Sequences from confirmation amplicon


Confirmation sequences for line 299E01 and hit At3g61300 are shown.
Line ID 299E01
Locus At3g61300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22688602
Primers used for PCR KU88 - 8474, show primer details
   
Sequence Name 04-K103453-0022-299-E01C-KU88
GenBank Accession LN487881 [GenBank]
Sequencing primer gene specific primer, KU88: 5'-AACGTTTAGATGAAGTCTCGTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K103453-0022-299-E01C-KU88
GTTTTAGACTAACAACGGTTAGGCACTGTCCGGTTATCGTCAATCCGTTTATCAATTGTA
CCAATCGGTATATGTACCTCTCCCAGCGTTTCCTCTCGGTTAGGTGCGACTCGGTCTTCA
ATCGAGATGATCAGATCTTCAAACGGTTCAGCCGCGACGAGAGTGAACTCTTCGTTCCAT
CTTGGATTCAGCGAGTGACTCGGTTTGGTTCGAACCACTTGGTTATTCAACCGGATTTTT
ACATATGGATTTGGCAATCGAGTCCGGTCTGGTACAATAACTAAGTCTTGTGCCTCGATG
ACATTAACCCGGAGGTACCAAAGCCTTGGAGAATGATAGACTTTAGAACGCAAACTCGAT
TTGTTAACAGCGTTTAAAGCGTCTGAGTATGTCGCGTCTGAGAACGCTTCGTCGGCTTGA
GTACCGAACCAAACAGCCAACATAATCTCGCCTCCTTTCTCGTTGTTGACTCTGTACCAT
TCTGGAGCTAAAGGACTGTCTGGTGCGACACGTGTCGGAATCTGATTGAGATCGAACCGG
ATTAAACCGACGAAATCGTCTTTGACCATGTAAATGGCTTCATACTTCAAGAACGACAAA
TGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATT
Primers used for PCR KU87 - 8474, show primer details
   
Sequence Name 01-K103453-0022-299-E01C-KU87
GenBank Accession LN486538 [GenBank]
Sequencing primer gene specific primer, KU87: 5'-AACATCCCCACAAATGAGAGTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103453-0022-299-E01C-KU87
CATCGTTCACCGCAACCGCCTCCGCCTCATCGCAAACGGCTCTACCGCCACCGATGATGG
AGTCTTCTCTCTATCAAGCACCGAGGTTTGGTACTCCGATACCTACAACTATGGGGTTTA
ATCCGAATCCACCGGATTATTCTATCAAAGAGACGAAACCGATTCTCGGAGGAGGCAAAC
GAGCAAGATCAAGTGATCATGATCTCGTCGAGCCAATGGAGTTTCTCTTCATCAAAATCG
TGAAAGCTAGAAATCTTCCATCCATGGACTTAACCGGAAGTCTAGATCCATACATCGAAG
TCAAACTCGGAAACTATACCGGCAAAACCAAACATTTTGAGAAGAATCAAAACCCGGTTT
GGAACGAAGTCTTCGCTTTCTCCAAATCCAATCAACAATCAAACGTTCTTGAAGTATGAT
GGATCAAAGACGATACCCAATTTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 17-K103454-0022-299-E01C-8409
GenBank Accession LN493815 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103454-0022-299-E01C-8409
TTACTGTAAATTGGGTATCGTCTTTGATCCATCATACTTCACGAACGTTTGATTGTTGAT
TGGATTTGGAGAAAGCGAAGACTTCGTTCCAAACCGGGTTTTGATTCTTCTCAAAATGTT
TGGTTTTGCCGGTATAGTTTCCGAGTTTGACTTCGATGTATGGATCTAGACTTCCGGTTA
AGTCCATGGATGGAAGATTTCTAGCTTTCACGATTTTGATGAAGAGAAACTCCATTGGCT
CGACGAGATCATGATCACTTGATCTTGCTCGTTTGCCTCCTCCGAGAATCGGTTTCGTCT
CTTTGATAGAATAATCCGGTGGATTCGGATTAAACCCCATAGTTGTAGGTATCGGAGTAC
CAAACCTCGGTGCTTGATAGAGAGAAGACTCCATCATCGGTGGCGGTAGAGCCGTTTGCG
ATTGAGGCGGAGGCGGTTGCGGTTGAAACGATTGCTGATTCCCTCGTGGGCTATGGTTAC
TCTCATTTGTGGGGATGT

Last Updated on 06.12.2018 16:11