Sequences from confirmation amplicon


Confirmation sequences for line 299E05 and hit At3g21620 are shown.
Line ID 299E05
Locus At3g21620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7611149
Primers used for PCR 42qa - 8474, show primer details
   
Sequence Name 40-K115305-0022-299-E05C-42qa
GenBank Accession LN503813 [GenBank]
Sequencing primer gene specific primer, 42qa: 5'-GGATCAATGTCAACGTAAAAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115305-0022-299-E05C-42qa
ATAAGCTTTTAGGCTTTTTGTGTTGGTTCCGTATTTGGGAAGGATCAATTATTGTGTTAA
AAGGAAATCAATTTTACCTTTTTAATTTTTTTGCACTTTTGTTTTTCAATATTGCTGAAT
GTTGACTAATAACTTATTTGATTTGTTTTAGATAAATTTGATGACCTCTATTTGATATTG
TTTTTTTTTTGTATATATATGATATAGCAAAAACCAAAGTAAACTTTGGAAAACTTCTTT
CTATTAATAAAAAAAAACGTTCGTAAAAAATCATAAAAATCTCATCACCACTTTTCACTA
AGTATTAGTATTACTAAGTAAAGGTTTCAGCAAAGCCGCTTTTAAACCTCCTTGAGCCAC
GTTTAGTCGCCACTAAATCCGGCGTCTTATCGGGCGCTGGCTCCTCCACAACCATCTCAT
TTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCAATTCAAAAATGTAAATTTC
   
Sequence Name 05-K115304-0022-299-E05C-8409
GenBank Accession LN488474 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115304-0022-299-E05C-8409
TTGAGATGGTTGTGGAGGAGCCAGCGCCCGATAAGACGCCGGATTTAGTGGCGACTAAAC
GTGGCTCAAGGAGGTTTAATAGCGGCTCTGCTGAAACCTTTACTTAGTAATACTAATACT
TAGTGAAAAGTGGTGATGAGATTTTTATGATTTTTTACGAACGTTTCTTCTTATTAATAG
AAAGAAGTTTTCCAAAGTTTACTTTGGTTTTTGCTATATCATATATATACAAAAAAAAAA
CAATATCAAATAGAGGTCATCAAATTTATCTAAAACAAATCAAATAAGTTATTAGTCAAC
ATTCAGCAATATTGAAAAACAAAAGTGCAAAAAAATTAAAAAGGTAAAATTGATTTCCTT
TTAACACAATAATTGATCCTTCCCAAATACGGAACCAACACAAAAAGCCTAAAAGCTTAT
CGAAAACAATTCAATACTCTCTTCTCCGGAATCTCTTTACCTTTTTACGT

Last Updated on 06.12.2018 16:11