Sequences from confirmation amplicon


Confirmation sequences for line 299G11 and hit At1g67390 are shown.
Line ID 299G11
Locus At1g67390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25246702
Primers used for PCR 15uj - 8474, show primer details
   
Sequence Name 53-K117671-0022-299-G11C-15uj
GenBank Accession LN508282 [GenBank]
Sequencing primer gene specific primer, 15uj: 5'-TTCTCCATTTTCTACTTTGCGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117671-0022-299-G11C-15uj
GACTTTTGTTTATAATTGGACTAAAGATCTAGAGAAAGTGCCAAACATGAACTACAATAT
TACATCTCATGCTCAGGTACAAGTACAAGCATATATATATATATAGAAAATGGTATTTTA
TATATTGCTTAACATATTTTGTTTTACATGAATTGAAGGAGAAAAAAAATATTGGACATG
AGTTCGTGGTGAGTGGAGATGCTAGCTACTTGCAAAAGCTTAAGTCTTTGAAGGTGGTCG
TGGATGTAACAAATTCGAGAGAAGTCCACATGCTAAGGGACATTTTAGTTGCTTGGAATG
GAGTATTGGAAGAGCTCCATATACTATTTAAGGTACTTTCATTTTATGCTTTAATTTTAG
TACTTAGAAGATTTGATCAGTTTTCTTCTATCCGGATACCATTGCTCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
A
   
Sequence Name 05-K117670-0022-299-G11C-8409
GenBank Accession LN488533 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117670-0022-299-G11C-8409
CATTTACATTGAATATATCCTGAGCAATGGTATCCGGATAGAAGAAAACTGATCAAATCT
TCTAAGTACTAAAATTAAAGCATAAAATGAAAGTACCTTAAATAGTATATGGAGCTCTTC
CAATACTCCATTCCAAGCAACTAAAATGTCCCTTAGCATGTGGACTTCTCTCGAATTTGT
TACATCCACGACCACCTTCAAAGACTTAAGCTTTTGCAAGTAGCTAGCATCTCCACTCAC
CACGAACTCATGTCCAATATTTTTTTTCTCCTTCAATTCATGTAAAACAAAATATGTTAA
GCAATATATAAAATACCATTTTCTATATATATATATATGCTTGTACTTGTACCTGAGCAT
GAGATGTAATATTGTAGTTCATGTTTGGCACTTTCTCTAGATCTTTAGTCCAATTATTAA
ACAAAAGTCTTGGTGCCTTGATGACAAAGTTTTGTTCCTTAGCAAACGCAAAGTAGAAAA

Last Updated on 06.12.2018 16:11